抗精神病药物可促进人诱导多能干细胞衍生的神经干细胞的神经分化。
Antipsychotics promote neural differentiation of human iPS cell-derived neural stem cells.
作者信息
Asada Minoru, Mizutani Shuki, Takagi Masatoshi, Suzuki Hidenori
机构信息
Department of Pharmacology, Graduate School of Medicine, Nippon Medical School, 1-1-5 Sendagi, Bunkyo-ku, Tokyo 113-8602, Japan.
Department of Pediatrics and Developmental Biology, Graduate School of Medicine, Tokyo Medical and Dental University, 1-5-45 Yushima, Bunkyo-ku, Tokyo 113-8519, Japan.
出版信息
Biochem Biophys Res Commun. 2016 Nov 25;480(4):615-621. doi: 10.1016/j.bbrc.2016.10.102. Epub 2016 Oct 26.
We investigated the effects of antipsychotics on human induced pluripotent stem cell (hiPSC)-derived neural stem cell (NSC) differentiation. Induction of NSCs from hiPSCs was performed using PSC neural induction medium. Induced NSCs were subsequently cultured in neural differentiation medium containing antipsychotics. Cultured cells were subjected to neural differentiation marker analysis. As previously shown in rodent cells, antipsychotics promoted neural differentiation compared with vehicle treatment. Atypical antipsychotics appear to possess more differentiation induction potential than typical ones. Most NSCs do not express dopamine D2 receptor; however, our in vitro study indicates the clinical potential of antipsychotics could include effects independent of monoamine receptor expression in NSCs. Our study shows NSCs derived from hiPSCs provide opportunity to investigate the underlying direct effect of antipsychotics treatment on NSCs.
我们研究了抗精神病药物对人诱导多能干细胞（hiPSC）来源的神经干细胞（NSC）分化的影响。使用PSC神经诱导培养基从hiPSC诱导生成NSC。随后将诱导生成的NSC在含有抗精神病药物的神经分化培养基中培养。对培养的细胞进行神经分化标志物分析。如先前在啮齿动物细胞中所示，与载体处理相比，抗精神病药物促进了神经分化。非典型抗精神病药物似乎比典型抗精神病药物具有更强的分化诱导潜力。大多数NSC不表达多巴胺D2受体；然而，我们的体外研究表明，抗精神病药物的临床潜力可能包括独立于NSC中单胺受体表达的效应。我们的研究表明，源自hiPSC的NSC为研究抗精神病药物治疗对NSC的潜在直接作用提供了机会。
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