Department of Biotechnology, Indian Institute of Technology, Kharagpur 721302, India.
Department of Biotechnology, Indian Institute of Technology, Kharagpur 721302, India.
Bioresour Technol. 2017 Jan;223:219-226. doi: 10.1016/j.biortech.2016.10.016. Epub 2016 Oct 19.
The present study investigates the effect of spent media of acetogenic dark fermentation for mixotrophic algal cultivation for biodiesel production. Mixotrophic growth conditions were optimized in culture flask (250mL) using Chlorella sp. MJ 11/11. Maximum lipid accumulation (58% w/w) was observed under light intensity, pH, nitrate and phosphate concentration of 100μmolms, 7, 2.7mM and 1.8mM, respectively. Air lift (1.4L) and flat panel (1.4L) reactors were considered for algal cultivation. Air lift showed significant improvement in biomass and lipid production as compared to flat panel reactor. The results could help in development of sustainable technology involving acetogenic hydrogen production integrated with sequential mitigation of spent media by algal cultivation for improved energy recovery.
本研究考察了用于混合营养藻类培养生产生物柴油的产乙酸发酵废培养基的效果。使用 Chlorella sp. MJ 11/11 在 250mL 培养瓶中优化了混合营养生长条件。在光强、pH 值、硝酸盐和磷酸盐浓度分别为 100μmolms、7、2.7mM 和 1.8mM 时,观察到最大脂质积累(58%w/w)。分别考虑了气升式(1.4L)和平板式(1.4L)反应器用于藻类培养。与平板式反应器相比,气升式反应器显著提高了生物量和脂质产量。这些结果有助于开发涉及产乙酸氢生产的可持续技术,该技术与通过藻类培养顺序减轻废培养基相结合,以提高能源回收。