Song Bo, Oehrle Nathan W, Liu Shanshan, Krishnan Hari B
Plant Genetics Research Unit, USDA-Agricultural Research Service , Columbia, Missouri 65211, United States.
Key Laboratory of Soybean Biology at the Chinese Ministry of Education, Northeast Agricultural University , Harbin, China.
J Agric Food Chem. 2016 Nov 16;64(45):8499-8508. doi: 10.1021/acs.jafc.6b03677. Epub 2016 Nov 8.
Perennial Glycine species, distant relatives of soybean, have been recognized as a potential source of new genetic diversity for soybean improvement. The subgenus Glycine includes around 30 perennial species, which are well-adapted to drought conditions and possess resistance to a number of soybean pathogens. In spite of the potential of the perennial Glycine species for soybean improvement, very little is known about their storage proteins and their relationship with cultivated soybean seed proteins. We have examined the seed protein composition of nine perennial Glycine species by one- and two-dimensional (1-D and 2-D) gel electrophoresis. The relationship between cultivated soybean and perennial soybean seed proteins was examined by immunoblot analyses using antibodies raised against G. max β-conglycinin, glycinin A3 subunit, lipoxygenase, leginsulin, Kunitz trypsin inhibitor, and Bowman-Birk protease inhibitor. Additionally, we have measured the trypsin and chymotrypsin inhibitor activities from cultivated soybean and perennial Glycine species and have found marked differences between them. Our 2-D gel and immunoblot analyses demonstrate significant differences in the protein composition and size heterogeneities of the 7S and 11S seed storage proteins of soybean and perennial Glycine species. Perennial Glycine species accumulated a 45 kDa protein that was not detected in G. max and G. soja. This unique 45 kDa protein was immunologically related to the A3 glycinin subunit of G. max. The results of our studies suggest that even though the seed proteins of wild perennial Glycine species and G. max are immunologically related, their genes have diverged from each other during the course of evolution.
多年生大豆属物种是大豆的远亲,已被公认为是用于改良大豆的新遗传多样性的潜在来源。大豆亚属包括约30个多年生物种,它们非常适应干旱条件,并且对多种大豆病原体具有抗性。尽管多年生大豆属物种在改良大豆方面具有潜力,但对于它们的贮藏蛋白以及它们与栽培大豆种子蛋白的关系却知之甚少。我们通过一维(1-D)和二维(2-D)凝胶电泳分析了9种多年生大豆属物种的种子蛋白组成。使用针对大豆β-伴大豆球蛋白、大豆球蛋白A3亚基、脂氧合酶、豆胰岛素、库尼兹胰蛋白酶抑制剂和鲍曼-伯克蛋白酶抑制剂产生的抗体,通过免疫印迹分析研究了栽培大豆和多年生大豆种子蛋白之间的关系。此外,我们测量了栽培大豆和多年生大豆属物种的胰蛋白酶和糜蛋白酶抑制剂活性,发现它们之间存在显著差异。我们的二维凝胶和免疫印迹分析表明,大豆和多年生大豆属物种的7S和11S种子贮藏蛋白在蛋白质组成和大小异质性方面存在显著差异。多年生大豆属物种积累了一种在大豆和野生大豆中未检测到的45 kDa蛋白质。这种独特的45 kDa蛋白质与大豆的A3大豆球蛋白亚基具有免疫相关性。我们的研究结果表明,尽管野生多年生大豆属物种和大豆的种子蛋白具有免疫相关性,但它们的基因在进化过程中已经彼此分化。