Han Weiqi, He Wei, Yang Wanlei, Li Jianlei, Yang Zhifan, Lu Xuanyuan, Qin An, Qian Yu
Department of Orthopaedics, Shaoxing People's Hospital (Shaoxing Hospital of Zhejiang University), Shaoxing, Zhejiang 312000, PR China.
Department of Orthopaedics, Shanghai Key Laboratory of Orthopaedic Implant, Shanghai Ninth People's Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200011, PR China.
Sci Rep. 2016 Oct 31;6:36330. doi: 10.1038/srep36330.
Bone callus, generated during fracture healing, is commonly discarded during surgical procedures. The aim of this study was to investigate the osteogenic potential of bone callus and its possible use as autograft material for patients needing bone grafts. Histology, immunohistochemistry, micro-computed tomography, and biomechanics were performed to examine osteogenic cells, osteoinductive factors, and the osteoconductive structure of bone callus. Alkaline phosphatase-positive osteoblasts, osteoinductive factors (including BMP2, FGF2, TGFB1, and IGF1), and a porous structure were found in bone callus. Early-stage callus (within 3 months after fracture) presented significantly improved osteogenic properties compared to medium- (3-9 months) and late-stage (longer than 9 months) callus. The results revealed that bone callus induced new bone formation in a nude mouse model. Early-stage callus showed better performance to medium- and late-stage callus in the induction of new bone formation at both 8 and 12 weeks. These findings indicated that bone callus, especially early-stage callus, possesses osteogenic potential and can potentially serve as an alternative source of material for bone grafts.
骨折愈合过程中产生的骨痂在外科手术中通常会被丢弃。本研究的目的是调查骨痂的成骨潜力及其作为需要骨移植患者的自体移植材料的可能性。进行了组织学、免疫组织化学、微计算机断层扫描和生物力学分析，以检查骨痂中的成骨细胞、骨诱导因子和骨传导结构。在骨痂中发现了碱性磷酸酶阳性成骨细胞、骨诱导因子（包括骨形态发生蛋白2、成纤维细胞生长因子2、转化生长因子β1和胰岛素样生长因子1）以及多孔结构。与中期（骨折后3-9个月）和后期（超过9个月）骨痂相比，早期骨痂（骨折后3个月内）的成骨特性显著改善。结果显示，骨痂在裸鼠模型中诱导了新骨形成。在8周和12周时，早期骨痂在诱导新骨形成方面比中期和后期骨痂表现更好。这些发现表明，骨痂，尤其是早期骨痂，具有成骨潜力，有可能作为骨移植的替代材料来源。
