一种用于体内Cdc14磷酸酶活性定量的新方法。

A New Methodology for the Quantification of In Vivo Cdc14 Phosphatase Activity.

作者信息

Queralt Ethel, Rodriguez-Rodriguez José-Antonio

机构信息

Cancer Epigenetics & Biology Program, IDIBELL,Hospitalet de Llobregat, Barcelona, Spain.

Molecular Biology Program, Sloan Kettering Institute, Memorial Sloan Kettering Cancer Center, New York, NY, 10065, USA.

出版信息

Methods Mol Biol. 2017;1505:89-96. doi: 10.1007/978-1-4939-6502-1_8.

DOI:10.1007/978-1-4939-6502-1_8

PMID:27826859

Abstract

The phosphatase Cdc14 has a pivotal function in the mitotic exit of Saccharomyces cerevisiae. During interphase, Cdc14 remains inactive in the nucleolus bound to the inhibitor Net1. Cdc14 activation occurs in the metaphase to anaphase transition and it is promoted by at least two signaling pathways called FEAR (CdcFourteen Early Anaphase Release) and MEN (Mitotic Exit Network). These two pathways act in parallel and target the phosphorylation of Net1, thus decreasing Net1 affinity for Cdc14. The activity of Cdc14 can be used as a readout to assay functional interactions of different components of the mitotic exit signaling pathways.

摘要

磷酸酶Cdc14在酿酒酵母的有丝分裂退出过程中起关键作用。在间期，Cdc14与抑制剂Net1结合，在核仁中保持无活性。Cdc14的激活发生在中期到后期的转变过程中，至少由两条称为FEAR（Cdc14早期后期释放）和MEN（有丝分裂退出网络）的信号通路促进。这两条通路并行作用，靶向Net1的磷酸化，从而降低Net1对Cdc14的亲和力。Cdc14的活性可作为一种读数，用于分析有丝分裂退出信号通路不同组分的功能相互作用。