Liu Yalei, Gong Yan, Qu Chenxue, Zhang Yang, You Ran, Yu Nan, Lu Guizhi, Huang Youyuan, Zhang Hong, Gao Ying, Gao Yanming, Guo Xiaohui
Department of Endocrinology, Peking University First Hospital, Beijing, 100034, PR China.
Department of Clinical Laboratory, Peking University First Hospital, Beijing, 100034, PR China.
Mol Cell Endocrinol. 2017 Jan 15;440:1-7. doi: 10.1016/j.mce.2016.11.004. Epub 2016 Nov 7.
Inhibitory CD32b receptors on B cells are critical for humoral immunity. The humoral response plays a role in the pathogenesis of Hashimoto's thyroiditis (HT). This study aimed to investigate B cell subset distribution and CD32b expression within these subsets in HT patients. B cell subset distribution and CD32b expression were analyzed in 60 HT patients and 21 healthy donors. Subset distribution and CD32b expression following stimulation with α-Ig and α-CD40 were also assessed. The percentage of double-negative (DN) memory cells was increased in the HT patients, while the expression level of CD32b on DN memory cells was decreased. Redistribution of B cell subsets was detected in response to stimulation with α-Ig. In addition, the expression level of CD32b was reduced following α-CD40 stimulation. These results suggest that abnormal B cell subset distribution and decreased CD32b expression on DN memory cells might be involved in the pathogenesis of HT.
B细胞上的抑制性CD32b受体对体液免疫至关重要。体液反应在桥本甲状腺炎（HT）的发病机制中起作用。本研究旨在调查HT患者中B细胞亚群分布以及这些亚群内CD32b的表达情况。分析了60例HT患者和21名健康供体的B细胞亚群分布及CD32b表达。还评估了用α-Ig和α-CD40刺激后的亚群分布及CD32b表达。HT患者中双阴性（DN）记忆细胞的百分比增加，而DN记忆细胞上CD32b的表达水平降低。检测到用α-Ig刺激后B细胞亚群发生了重新分布。此外，α-CD40刺激后CD32b的表达水平降低。这些结果表明，异常的B细胞亚群分布以及DN记忆细胞上CD32b表达降低可能参与了HT的发病机制。
