Liu Ying, Xia Yan, Rad Armin Tahmasbi, Aresh Wafa, Nieh Mu-Ping
Department of Chemical and Biomolecular Engineering, University of Connecticut, Storrs, CT, 06269, USA.
Department of Biomedical Engineering, University of Connecticut, Storrs, CT, 06269, USA.
Methods Mol Biol. 2017;1522:273-282. doi: 10.1007/978-1-4939-6591-5_22.
Bicellar mixtures have been used as alignable membrane substrates for the structural characterization of membrane-associated proteins. Most recently, it has been shown that bicelles can serve as nanocarriers to effectively deliver hydrophobic molecules to cancer cells with a 3- to 10-fold enhancement compared to that of chemically identical liposomes. In this chapter, a detailed preparation protocol, common structural characterization methods, the structural stability and the cellular uptake of bicellar nanodisks are discussed.
双分子层混合物已被用作可排列的膜底物，用于膜相关蛋白的结构表征。最近的研究表明，双分子层囊泡可以作为纳米载体，与化学组成相同的脂质体相比，能以3至10倍的效率将疏水分子有效递送至癌细胞。在本章中，将讨论双分子层纳米盘的详细制备方案、常见的结构表征方法、结构稳定性以及细胞摄取情况。
