Tanese N, Roth M, Epstein H, Goff S P
Department of Biochemistry, Columbia University, College of Physicians and Surgeons, New York, New York 10032.
Virology. 1989 Jun;170(2):378-84. doi: 10.1016/0042-6822(89)90428-5.
An insertion mutation in the pol gene of Moloney murine leukemia virus (M-MuLV) was found to render the virus temperature-sensitive for replication. A provirus containing a 12-bp insertion at the boundary between the reverse transcriptase (RT) and integrase (IN) domains induced the formation of mutant virions containing a partially processed RT-IN fusion protein. Some proteolytic processing to form mature RT and IN was observed at 32 degrees, but only aberrantly processed proteins were detected at 39 degrees. The uncleaved precursor was found to exhibit DNA polymerase activity, even though it could not support replication of the virus in vivo at 39 degrees.
发现莫洛尼鼠白血病病毒（M-MuLV）的pol基因中的一个插入突变使该病毒在复制方面具有温度敏感性。一个在前逆转录酶（RT）和整合酶（IN）结构域之间的边界处含有12个碱基对插入的前病毒诱导形成了含有部分加工的RT-IN融合蛋白的突变病毒粒子。在32摄氏度时观察到一些蛋白水解加工以形成成熟的RT和IN，但在39摄氏度时仅检测到异常加工的蛋白质。发现未切割的前体具有DNA聚合酶活性，尽管它在39摄氏度时不能在体内支持病毒的复制。
