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使用促卵泡素或普乐赛进行超数排卵后奶牛的胚胎产量

Embryo yield in dairy cattle after superovulation with Folltropin or Pluset.

作者信息

Mikkola M, Taponen J

机构信息

Department of Production Animal Medicine, University of Helsinki, Saarentaus, Finland; VikingGenetics Finland, Hollola, Finland.

Department of Production Animal Medicine, University of Helsinki, Saarentaus, Finland.

出版信息

Theriogenology. 2017 Jan 15;88:84-88. doi: 10.1016/j.theriogenology.2016.09.052. Epub 2016 Oct 6.

Abstract

Two commercial FSH products were compared in a retrospective study on 3990 commercial superovulations and embryo recoveries in dairy heifers and cows. In addition, the 56-day nonreturn rate of 19,400 embryos produced with these two preparations was analyzed. Embryo collections were performed during a 16-year period from donors of Holstein and Ayrshire breeds. Folltropin (Vetoquinol S.A., Lure cedex, France) group (Group F) consisted of 2592 superovulations, of which 80% were performed on heifers and 20% on cows, and Pluset (Laboratorios Calier, S.A., Barcelona, Spain) group (Group P) of 1398 treatments, of which 66% and 34% were on heifers and cows, respectively. Total number of recovered structures, number of transferable embryos, and the proportion of unfertilized ova (UFO) and degenerated embryos were analyzed. Distribution of embryos into quality grades (1-3) and developmental stages (4-9) according to the IETS classification guidelines and means for each collection were evaluated. The proportion of low-responders having fewer than five corpora lutea and yielding fewer than five embryos or ova was investigated for each treatment. Group P yielded 1.1 recovered structures more than Group F (P < 0.001). Consequently, however, the number of transferable embryos did not differ among the groups, being 7.0 and 7.1 in Groups F and P, respectively. Instead, there was an increase in the number of UFO from 2.0 in Group F to 3.0 in Group P (P < 0.001). The quality of embryos and the developmental stages were similar between the groups and there was no difference in the proportion of low-responding donors in Group F and Group P. Also, there was no difference in the nonreturn rate after transfer of embryos originating from donors superovulated with Folltropin or Pluset. It was concluded that equal numbers of transferable embryos and pregnancies can be achieved with Folltropin and Pluset.

摘要

在一项回顾性研究中,对两种商业促卵泡素(FSH)产品在3990例奶牛和小母牛的商业性超数排卵及胚胎回收情况进行了比较。此外,还分析了用这两种制剂产生的19400个胚胎的56天不返情率。胚胎采集在16年期间内对荷斯坦和艾尔郡品种的供体进行。促卵泡素组(法国卢尔塞德维克托昆诺公司的Folltropin,F组)包括2592次超数排卵,其中80%在小母牛上进行,20%在母牛上进行;普乐赛特组(西班牙巴塞罗那卡利尔实验室的Pluset,P组)有1398次处理,其中66%在小母牛上进行,34%在母牛上进行。分析了回收结构的总数、可移植胚胎的数量、未受精卵(UFO)和退化胚胎的比例。根据国际胚胎移植协会(IETS)的分类指南,评估了胚胎按质量等级(1 - 3级)和发育阶段(4 - 9级)的分布情况以及每次采集的平均值。研究了每种处理中黄体少于5个且产生胚胎或卵子少于5个的低反应者的比例。P组比F组多回收1.1个结构(P < 0.001)。然而,两组之间可移植胚胎的数量没有差异,F组和P组分别为7.0和7.1。相反,UFO的数量从F组的2.0增加到P组的3.0(P < 0.001)。两组之间胚胎质量和发育阶段相似,F组和P组中低反应供体的比例没有差异。此外,用促卵泡素或普乐赛特进行超数排卵的供体所产生的胚胎移植后的不返情率也没有差异。得出的结论是,使用促卵泡素和普乐赛特可获得数量相等的可移植胚胎和妊娠。

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