Souza Livia T A, Mendes Adriano A, de Castro Heizir F
Department of Chemical Engineering, Engineering School of Lorena, University of São Paulo, 12602-810 Lorena, SP, Brazil.
Institute of Chemistry, Federal University of Alfenas, 37130-001 Alfenas, MG, Brazil.
Biomed Res Int. 2016;2016:1404567. doi: 10.1155/2016/1404567. Epub 2016 Oct 27.
The present study deals with the enzymatic synthesis of biodiesel by transesterification of Jatropha oil ( L.) with ethanol in a solvent-free system. Seven commercial lipase preparations immobilized by covalent attachment on epoxy-polysiloxane-polyvinyl alcohol composite (epoxy-SiO-PVA) were tested as biocatalysts. Among them, immobilized lipases from (lipase AK) and (lipase PS) were the most active biocatalysts in biodiesel synthesis, reaching ethyl ester yields (FAEE) of 91.1 and 98.3% at 72 h of reaction, respectively. The latter biocatalyst exhibited similar performance compared to Novozym® 435. Purified biodiesel was characterized by different techniques. Transesterification reaction carried out under microwave irradiation exhibited higher yield and productivity than conventional heating. The operational stability of immobilized lipase PS was determined in repeated batch runs under conventional and microwave heating systems, revealing half-life times of 430.4 h and 23.5 h, respectively.
本研究涉及在无溶剂体系中通过麻风树油与乙醇进行酯交换反应酶法合成生物柴油。测试了七种通过共价连接固定在环氧 - 聚硅氧烷 - 聚乙烯醇复合材料（epoxy - SiO - PVA）上的商业脂肪酶制剂作为生物催化剂。其中，来自[具体来源1]的固定化脂肪酶（脂肪酶AK）和[具体来源2]的固定化脂肪酶（脂肪酶PS）是生物柴油合成中活性最高的生物催化剂，在反应72小时时分别达到91.1%和98.3%的脂肪酸乙酯产率（FAEE）。后一种生物催化剂与诺维信435表现出相似的性能。通过不同技术对纯化的生物柴油进行了表征。在微波辐射下进行的酯交换反应比传统加热表现出更高的产率和生产率。在传统和微波加热系统下的重复批次运行中测定了固定化脂肪酶PS的操作稳定性，结果表明其半衰期分别为430.4小时和23.5小时。
