将艾芬地尔注入大鼠喙部前扣带回皮质对骨癌痛大鼠疼痛相关厌恶的影响。

Effect of the ifenprodil administered into rostral anterior cingulate cortex on pain-related aversion in rats with bone cancer pain.

作者信息

Feng Hao, Chen Zhaowei, Wang Gongming, Zhao Xin, Liu Zhonghao

机构信息

Department of Anesthesiology, the Second Hospital of Shandong University, Jinan, China.

Plastic surgery, Liaocheng People's Hospital, Liaocheng, China.

出版信息

BMC Anesthesiol. 2016 Nov 21;16(1):117. doi: 10.1186/s12871-016-0283-1.

DOI:10.1186/s12871-016-0283-1

PMID:27871230

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5117499/

Abstract

BACKGROUND

To assess the effect of rostral anterior cingulate cortex (rACC) administration with ifenprodil (NR2B receptor blocker) on bone cancer pain (BCP)-related aversion sentiment using the conditioned place avoidance experiments in rats.

METHODS

A total of 30 male Wistar rats without place preference were randomly assigned to three groups: control group (Group C, n = 10), BCP group (Group P, n = 10) and ifenprodil group (Group Ifen, n = 10). Three microliter MADB-106 cells were inoculated into right tibia bone marrow cavity in group P and Ifen, while the same dose of normal saline in group C as a control. Ifenprodil was administered into the rACC at the 14th day after inoculation in group Ifen and normal saline in group C and P. Mechanical stimulation pain thresholds of the rats' right hind paws were measured using Von Frey stimulation method at 1 day before injection of the tumor cells and at 3, 7,10, 12 and 14 days after the injection. The pain-related aversion in rats with BCP was determined by the conditioned place avoidance (CPA) test at 14 days after injection of ifenprodil.

RESULTS

The mechanical stimulation pain thresholds substantially decreased in rats in groups P and Ifen from 10 days to 14 days after the incubation with the MADB-106 cells (P < 0.05). There were significant differences in pain thresholds in groups P and Ifen compared to group C at 10, 12 and 14 days after inoculation (P < 0.05). The percentage of residence time in chamber A was (30 ± 4%) in group P, which was lower than (52 ± 5%) in group C (P < 0.05). After ifenprodil treatment, the percentage time in chamber A increased to (42 ± 5%), which was higher than that in group P and still lower than that in group C (P < 0.05).

CONCLUSION

Ifenprodil administered into rACC as a selective NR2B antagonist can effectively alleviate pain-related aversion sentiment in rats with BCP.

摘要

背景

采用大鼠条件性位置回避实验，评估向喙前部扣带回皮质（rACC）注射ifenprodil（NR2B受体阻滞剂）对骨癌疼痛（BCP）相关厌恶情绪的影响。

方法

将30只无位置偏好的雄性Wistar大鼠随机分为三组：对照组（C组，n = 10）、BCP组（P组，n = 10）和ifenprodil组（Ifen组，n = 10）。P组和Ifen组将3微升MADB - 106细胞接种到右胫骨骨髓腔，C组接种相同剂量的生理盐水作为对照。Ifen组在接种后第14天向rACC注射ifenprodil，C组和P组注射生理盐水。在注射肿瘤细胞前1天以及注射后3、7、10、12和14天，采用von Frey刺激法测量大鼠右后爪的机械刺激疼痛阈值。在注射ifenprodil后14天，通过条件性位置回避（CPA）试验确定BCP大鼠的疼痛相关厌恶情绪。

结果

接种MADB - 106细胞后10天至14天，P组和Ifen组大鼠的机械刺激疼痛阈值显著降低（P < 0.05）。接种后10、12和14天，P组和Ifen组的疼痛阈值与C组相比有显著差异（P < 0.05）。P组在A室的停留时间百分比为（30 ± 4%），低于C组的（52 ± 5%）（P < 0.05）。ifenprodil治疗后，A室的停留时间百分比增加到（42 ± 5%），高于P组但仍低于C组（P < 0.05）。