The Oxidative and Inflammatory State in Patients with Acute Renal Graft Dysfunction Treated with Tacrolimus.

To determine the oxidative stress/inflammation behavior in patients with/without acute graft dysfunction (AGD) with Tacrolimus. Cross-sectional study, in renal transplant (RT) recipients (1-yr follow-up). Patients with AGD and without AGD were included. Serum IL-6, TNF-, 8-isoprostanes (8-IP), and Nitric Oxide (NO) were determined by ELISA; C-reactive protein (CRP) was determined by nephelometry; lipid peroxidation products (LPO) and superoxide dismutase (SOD) were determined by colorimetry. The AGD presentation was at 5.09 ± 3.07 versus 8.27 ± 3.78 months ( < 0.001); CRP >3.19 mg/L was found in 21 versus 19 in the N-AGD group ( = 0.83); TNF- 145.53 ± 18.87 pg/mL versus 125.54 ± 15.92 pg/mL in N-AGD ( = 0.64); IL-6 2110.69 ± 350.97 pg/mL versus 1933.42 ± 235.38 pg/mL in N-AGD ( = 0.13). The LPO were higher in AGD ( = 0.014): 4.10 ± 0.69 M versus 2.41 ± 0.29 M; also levels of 8-IP were higher in AGD 27.47 ± 9.28 pg/mL versus 8.64 ± 1.54 pg/mL ( = 0.01). Serum levels of NO in AGD were lower 138.44 ± 19.20 mol/L versus 190.57 ± 22.04 mol/L in N-AGD ( = 0.042); antioxidant enzyme SOD activity was significantly diminished in AGD with 9.75 ± 0.52 U/mL versus 11.69 ± 0.55 U/mL in N-AGD ( = 0.012). Patients with RT present with a similar state of the proinflammatory cytokines whether or not they have AGD. The patients with AGD showed deregulation of the oxidative state with increased LPO and 8-IP and decreased NO and SOD.