Inoue Motoki, Takayanagi Mariko, Fujiu Katsuhito, Manabe Ichiro, Nagai Ryozo, Taguchi Tetsushi
Biomaterials Unit, Nano-Life Field, International Center for Materials Nanoarchitectonics (MANA), National Institute for Materials Science (NIMS), 1-1 Namiki, Tsukuba, Ibaraki, 305-0044, Japan.
Department of Cardiovascular Medicine, Graduate School of Medicine, The University of Tokyo, Tokyo, 113-8655, Japan.
Sci Technol Adv Mater. 2012 Nov 23;13(6):064208. doi: 10.1088/1468-6996/13/6/064208. eCollection 2012 Dec.
Tamibarotene-loaded biodegradable matrices with antithrombogenic and drug-releasing properties were prepared in a crosslinking reaction between amino groups of alkali-treated collagen (AlCol) and active ester groups of trisuccinimidyl citrate. The resulting matrices were characterized by their residual amino group concentrations, swelling ratios and thermal, antithrombogenic and drug-releasing properties. It was clarified that the addition of tamibarotene does not inhibit matrix formation. After immersion in water, the swelling ratio of a matrix became lower than that prior to immersion. Thermal analysis indicated that AlCol interacted with tamibarotene. The addition of tamibarotene to the matrix did not influence the antithrombogenic property of the resulting matrix. A matrix with a high crosslinking density had a prolonged tamibarotene elution time. These results demonstrate that tamibarotene-loaded matrices have great potential as a coating material for drug-eluting stents.
通过碱处理胶原蛋白（AlCol）的氨基与柠檬酸三琥珀酰亚胺酯的活性酯基团之间的交联反应，制备了具有抗血栓形成和药物释放特性的载他米巴罗汀可生物降解基质。通过其残余氨基浓度、溶胀率以及热、抗血栓形成和药物释放特性对所得基质进行了表征。结果表明，添加他米巴罗汀并不抑制基质形成。基质浸入水中后，其溶胀率低于浸入前。热分析表明AlCol与他米巴罗汀相互作用。向基质中添加他米巴罗汀不会影响所得基质的抗血栓形成特性。具有高交联密度的基质具有延长的他米巴罗汀洗脱时间。这些结果表明，载他米巴罗汀的基质作为药物洗脱支架的涂层材料具有巨大潜力。
