• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

新型雌激素受体生物传感器的纳米生物工程与表征

Nanobioengineering and Characterization of a Novel Estrogen Receptor Biosensor.

作者信息

Berthier Alexandre, Elie-Caille Céline, Lesniewska Eric, Delage-Mourroux Régis, Boireau Wilfrid

机构信息

Institut FEMTO-ST, Université de Franche Comté, Clinical-Innovation Proteomic Platform, CNRS - 25044 Besançon cedex, France.

Estrogènes, Expression Génique et Pathologies du Système Nerveux Central, EA3922, IFR 133, Université de Franche-Comté, UFR Sciences et Techniques, 16 route de Gray, 25030 Besançon cedex, France.

出版信息

Sensors (Basel). 2008 Jul 28;8(7):4413-4428. doi: 10.3390/s8074413.

DOI:10.3390/s8074413
PMID:27879944
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3697183/
Abstract

We constructed an original supramolecular assembly on a surface of sensor composed of an innovative combination of an engineered cytochrome b5 and a modified nucleic acid bound to a synthetic lipid hemimembrane. The protein/DNA block, called (PDNA) 2, was synthesized and purified before its immobilization onto a hybrid bilayer reconstituted on a gold surface. Surface plasmon resonance (SPR) and atomic force microscopy (AFM) were engaged in parallel on the same substrates in order to better understand dynamic events that occur at the surface of the biosensor. Good correlations were obtained in terms of specificity and reversibility. These findings allow us to present a first application of such biosensor in the study of the interaction processes between nuclear receptor and DNA.

摘要

我们在传感器表面构建了一种原始的超分子组装体，该组装体由工程化细胞色素b5与结合到合成脂质半膜上的修饰核酸的创新组合构成。名为(PDNA)2的蛋白质/DNA嵌段在固定到在金表面重构的混合双层之前进行了合成和纯化。表面等离子体共振(SPR)和原子力显微镜(AFM)在同一底物上并行使用，以便更好地理解生物传感器表面发生的动态事件。在特异性和可逆性方面获得了良好的相关性。这些发现使我们能够展示这种生物传感器在核受体与DNA相互作用过程研究中的首次应用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/501a/3697183/c82a66e0136e/sensors-08-04413f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/501a/3697183/862d4f8b5fdc/sensors-08-04413f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/501a/3697183/899b05b34903/sensors-08-04413f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/501a/3697183/eeba60cfed32/sensors-08-04413f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/501a/3697183/c82a66e0136e/sensors-08-04413f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/501a/3697183/862d4f8b5fdc/sensors-08-04413f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/501a/3697183/899b05b34903/sensors-08-04413f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/501a/3697183/eeba60cfed32/sensors-08-04413f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/501a/3697183/c82a66e0136e/sensors-08-04413f4.jpg

相似文献

1
Nanobioengineering and Characterization of a Novel Estrogen Receptor Biosensor.新型雌激素受体生物传感器的纳米生物工程与表征
Sensors (Basel). 2008 Jul 28;8(7):4413-4428. doi: 10.3390/s8074413.
2
Label-free sensing and atomic force spectroscopy for the characterization of protein-DNA and protein-protein interactions: application to estrogen receptors.无标记传感和原子力光谱法用于蛋白质-DNA 和蛋白质-蛋白质相互作用的表征：在雌激素受体中的应用。
J Mol Recognit. 2011 May-Jun;24(3):429-35. doi: 10.1002/jmr.1106.
3
Surface plasmon resonance biosensor for dopamine using D3 dopamine receptor as a biorecognition molecule.以D3多巴胺受体作为生物识别分子的用于多巴胺的表面等离子体共振生物传感器。
Biosens Bioelectron. 2007 Oct 31;23(3):421-7. doi: 10.1016/j.bios.2007.05.004. Epub 2007 Jun 2.
4
Determination of binding capacity and adsorption enthalpy between Human Glutamate Receptor (GluR1) peptide fragments and kynurenic acid by surface plasmon resonance experiments. Part 2: Interaction of GluR1270-300 with KYNA.通过表面等离子体共振实验测定人谷氨酸受体（GluR1）肽片段与犬尿喹啉酸之间的结合能力和吸附焓。第2部分：GluR1270 - 300与犬尿喹啉酸的相互作用
Colloids Surf B Biointerfaces. 2015 Sep 1;133:66-72. doi: 10.1016/j.colsurfb.2015.04.044. Epub 2015 Jun 4.
5
Dendrimer-functionalized self-assembled monolayers as a surface plasmon resonance sensor surface.树枝状聚合物功能化自组装单分子层作为表面等离子体共振传感器表面。
Langmuir. 2004 Aug 3;20(16):6808-17. doi: 10.1021/la0495276.
6
[Optical surface plasmon resonance biosensors in molecular fishing].[分子垂钓中的光学表面等离子体共振生物传感器]
Biomed Khim. 2015 Mar-Apr;61(2):231-8. doi: 10.18097/PBMC20156102231.
7
A spectroscopic and surface plasmon resonance study of oleic acid/DNA complexes.油酸/DNA复合物的光谱学与表面等离子体共振研究
J Biomol Struct Dyn. 2002 Oct;20(2):231-42. doi: 10.1080/07391102.2002.10506839.
8
Quantitative Investigation of Protein-Nucleic Acid Interactions by Biosensor Surface Plasmon Resonance.利用生物传感器表面等离子体共振对蛋白质-核酸相互作用进行定量研究。
Methods Mol Biol. 2015;1334:313-32. doi: 10.1007/978-1-4939-2877-4_20.
9
Unique supramolecular assembly of a redox protein with nucleic acids onto hybrid bilayer: towards a dynamic DNA chip.一种氧化还原蛋白与核酸在混合双分子层上形成的独特超分子组装体：迈向动态DNA芯片。
Biosens Bioelectron. 2005 Feb 15;20(8):1631-7. doi: 10.1016/j.bios.2004.07.015.
10
Studies of gold nanorod-iron oxide nanohybrids for immunoassay based on SPR biosensor.基于表面等离子体共振生物传感器的用于免疫分析的金纳米棒-氧化铁纳米杂化物的研究
Talanta. 2014 Jul;125:29-35. doi: 10.1016/j.talanta.2014.02.036. Epub 2014 Feb 27.

引用本文的文献

1
Molecular and nanoscale evaluation of N-cadherin expression in invasive bladder cancer cells under control conditions or GW501516 exposure.在对照条件或 GW501516 暴露下，对浸润性膀胱癌细胞中 N-钙黏蛋白表达的分子和纳米级评估。
Mol Cell Biochem. 2020 Aug;471(1-2):113-127. doi: 10.1007/s11010-020-03771-1. Epub 2020 Jun 9.
2
Oriented immobilization of His-tagged protein on a redox active thiol derivative of DPTA-Cu(II) layer deposited on a gold electrode--the base of electrochemical biosensors.氧化还原活性的 DPTA-Cu(II) 衍生物在金电极上沉积的 His 标记蛋白的定向固定——电化学生物传感器的基础。
Sensors (Basel). 2013 Sep 3;13(9):11586-602. doi: 10.3390/s130911586.

本文引用的文献

1
PDNA as building blocks for membrane-guided self-assemblies.作为膜引导自组装构建块的聚脱氧核糖核酸
Biochem Soc Trans. 2007 Jun;35(Pt 3):495-7. doi: 10.1042/BST0350495.
2
Construction of hybrid bilayer membrane (HBM) Biochips and characterization of the cooperative binding between cytochrome-c and HBM.混合双层膜（HBM）生物芯片的构建以及细胞色素-c与HBM之间协同结合的表征。
Langmuir. 2007 Jun 5;23(12):6835-42. doi: 10.1021/la063300o. Epub 2007 May 11.
3
Understanding ligand binding effects on the conformation of estrogen receptor alpha-DNA complexes: a combinational quartz crystal microbalance with dissipation and surface plasmon resonance study.
了解配体结合对雌激素受体α-DNA复合物构象的影响：结合耗散型石英晶体微天平与表面等离子体共振的研究
Biophys J. 2007 Jun 15;92(12):4415-23. doi: 10.1529/biophysj.106.099382. Epub 2007 Mar 23.
4
Characterization of protein--DNA interactions using surface plasmon resonance spectroscopy with various assay schemes.使用表面等离子体共振光谱和各种检测方案对蛋白质 - DNA 相互作用进行表征。
Biochemistry. 2007 Feb 27;46(8):2127-35. doi: 10.1021/bi061903t. Epub 2007 Feb 1.
5
Evaluation of two- and three-dimensional streptavidin binding platforms for surface plasmon resonance spectroscopy studies of DNA hybridization and protein-DNA binding.用于DNA杂交和蛋白质-DNA结合的表面等离子体共振光谱研究的二维和三维链霉亲和素结合平台的评估。
Biosens Bioelectron. 2007 May 15;22(11):2700-6. doi: 10.1016/j.bios.2006.11.012. Epub 2007 Jan 12.
6
Combinational application of surface plasmon resonance spectroscopy and quartz crystal microbalance for studying nuclear hormone receptor-response element interactions.表面等离子体共振光谱法与石英晶体微天平联用研究核激素受体 - 反应元件相互作用
Anal Chem. 2006 Aug 1;78(15):5552-8. doi: 10.1021/ac0606103.
7
Diffusive dynamics of vesicles tethered to a fluid supported bilayer by single-particle tracking.通过单粒子追踪研究 tethered 于流体支撑双层膜上的囊泡的扩散动力学。 （注：“tethered”此处不太明确其准确中文表述，暂保留英文，可根据具体专业语境进一步确定合适的中文词，比如“ tethered”可译为“ tethered （拴系、束缚等意思，此处需结合专业知识准确表述）于” ）
Langmuir. 2006 Jun 20;22(13):5682-9. doi: 10.1021/la0534219.
8
Polymer-supported membranes as models of the cell surface.聚合物支撑膜作为细胞表面模型。
Nature. 2005 Sep 29;437(7059):656-63. doi: 10.1038/nature04164.
9
Scanning probe microscopic studies of the oriented attachment and membrane reconstitution of cytochrome C oxidase to a gold electrode.细胞色素C氧化酶在金电极上的定向附着和膜重构的扫描探针显微镜研究。
Langmuir. 2005 Sep 13;21(19):8580-3. doi: 10.1021/la051195x.
10
Gravimetric antigen detection utilizing antibody-modified lipid bilayers.利用抗体修饰脂质双层进行重量法抗原检测。
Anal Biochem. 2005 Oct 1;345(1):72-80. doi: 10.1016/j.ab.2005.05.031.