Yokoi Michinori, Shimoda Mitsuya
Tobacco Science Research Center, Japan Tobacco Inc., 6-2, Umegaoka, Aoba-ku, Yokohama227-8512, Japan.
Food Process Engineering, Division of Food Science and Technology, Bioresource and Bioenvironmental Science, Kyushu University, 6-10-1, Hakozaki, Higashi-ku, Fukuoka812-8581, Japan.
J Chromatogr Sci. 2017 Mar 1;55(3):373-377. doi: 10.1093/chromsci/bmw178.
A low-density polyethylene (LDPE) membrane pouch method was developed to extract volatile flavor compounds from tobacco leaf. Tobacco leaf suspended in water was enclosed in a pouch prepared from a LDPE membrane of specific gravity 0.92 g/cm3 and 0.03 mm thickness and then extracted with diethyl ether. In comparison with direct solvent extraction, LDPE membrane excluded larger and higher boiling point compounds which could contaminate a gas chromatograph inlet and damage a column. Whilst being more convenient than a reduced-pressure steam distillation, it could extract volatile flavor compounds of wide range of molecular weight and polarity. Repeatabilities in the extracted amounts were ranged from 0.38% of 2.3-bipyridyl to 26% of β-ionone, and average value of 39 compounds was 5.9%.
开发了一种低密度聚乙烯（LDPE）膜袋法从烟叶中提取挥发性风味化合物。将悬浮在水中的烟叶装入由比重为0.92 g/cm³、厚度为0.03 mm的LDPE膜制成的袋子中，然后用乙醚萃取。与直接溶剂萃取相比，LDPE膜排除了可能污染气相色谱仪进样口并损坏色谱柱的较大和较高沸点的化合物。虽然它比减压蒸汽蒸馏更方便，但它可以提取广泛分子量和极性的挥发性风味化合物。提取物量的重复性范围为2,3-联吡啶的0.38%至β-紫罗兰酮的26%，39种化合物的平均值为5.9%。
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