Tobias Nicholas J, Ahrendt Tilman, Schell Ursula, Miltenberger Melissa, Hilbi Hubert, Bode Helge B
Fachbereich Biowissenschaften, Merck Stiftungsprofessur für Molekulare Biotechnologie, Goethe Universität , Frankfurt am Main , Germany.
Max von Pettenkofer Institute, Ludwig-Maximilians-Universität München , Munich , Germany.
PeerJ. 2016 Nov 24;4:e2720. doi: 10.7717/peerj.2720. eCollection 2016.
Several members of the genus cause Legionnaires' disease, a potentially debilitating form of pneumonia. Studies frequently focus on the abundant number of virulence factors present in this genus. However, what is often overlooked is the role of secondary metabolites from . Following whole genome sequencing, we assembled and annotated the DSM 19216 genome. Together with 14 other members of the , we performed comparative genomics and analysed the secondary metabolite potential of each strain. We found that contains a huge variety of biosynthetic gene clusters (BGCs) that are potentially making a significant number of novel natural products with undefined function. Surprisingly, only a single Sfp-like phosphopantetheinyl transferase is found in all strains analyzed that might be responsible for the activation of all carrier proteins in primary (fatty acid biosynthesis) and secondary metabolism (polyketide and non-ribosomal peptide synthesis). Using conserved active site motifs, we predict some novel compounds that are probably involved in cell-cell communication, differing to known communication systems. We identify several gene clusters, which may represent novel signaling mechanisms and demonstrate the natural product potential of .
该属的几个成员会引发军团病,这是一种可能使人衰弱的肺炎形式。研究通常聚焦于该属中存在的大量毒力因子。然而,常常被忽视的是该属次生代谢产物的作用。在完成全基因组测序后,我们组装并注释了嗜肺军团菌DSM 19216的基因组。我们与嗜肺军团菌属的其他14个成员一起进行了比较基因组学研究,并分析了每个菌株的次生代谢产物潜力。我们发现嗜肺军团菌包含大量的生物合成基因簇(BGCs),这些基因簇可能会产生大量功能未知的新型天然产物。令人惊讶的是,在所有分析的嗜肺军团菌菌株中仅发现了一种类似Sfp的磷酸泛酰巯基乙胺基转移酶,它可能负责激活初级代谢(脂肪酸生物合成)和次级代谢(聚酮化合物和非核糖体肽合成)中的所有载体蛋白。利用保守的活性位点基序,我们预测了一些可能参与细胞间通讯的新型化合物,这与已知的通讯系统不同。我们鉴定出了几个基因簇,它们可能代表了新型信号传导机制,并证明了嗜肺军团菌的天然产物潜力。