Marafie H M, el-Ezaby M S, Fareed S
Chemistry Department, Kuwait University, Safat.
J Inorg Biochem. 1989 Sep;37(1):7-16. doi: 10.1016/0162-0134(89)80024-8.
The interaction of Cu(II) with pyridoxamine-5'-phosphate (PMP) and pyridoxal-5'-phosphate (PLP) was studied potentiometrically. The titration data were assessed by MINIQUAD program. Several protonated and nonprotonated complexes have been found to exist in solution. The reaction of PLP with Cu(II)-PMP has been studied kinetically, using the stopped-flow technique. Two rate steps have been observed. The first step has been attributed to the formation of a Schiff's base metal complex. The second step may be due to the formation of a ternary complex formation. A mechanism was suggested.
通过电位滴定法研究了铜（II）与磷酸吡哆胺-5'-磷酸酯（PMP）和磷酸吡哆醛-5'-磷酸酯（PLP）的相互作用。滴定数据通过MINIQUAD程序进行评估。已发现溶液中存在几种质子化和非质子化的配合物。采用停流技术对PLP与Cu（II）-PMP的反应进行了动力学研究。观察到两个速率步骤。第一步归因于席夫碱金属配合物的形成。第二步可能是由于三元配合物的形成。提出了一种机理。
