Nutrition and Food Science, Universitat de València, Avda. Vicent Andrés Estellés, s/n 46100 Burjassot, València, Spain.
Food Chem. 2017 Apr 15;221:1834-1842. doi: 10.1016/j.foodchem.2016.10.093. Epub 2016 Oct 26.
A comparative study of the bioaccessibility of bioactive compounds and antioxidant capacity in a fruit juice-Stevia rebaudiana mixture processed by pulsed electric fields (PEF), high voltage electrical discharges (HVED) and ultrasound (USN) technology at two equivalent energy inputs (32-256kJ/kg) was made using an in vitro model. Ascorbic acid was not detected following intestinal digestion, while HVED, PEF and USN treatments increased total carotenoid bioaccessibility. HVED at an energy input of 32kJ/kg improved bioaccessibility of phenolic compounds (34.2%), anthocyanins (31.0%) and antioxidant capacity (35.8%, 29.1%, 31.9%, for TEAC, ORAC and DPPH assay, respectively) compared to untreated sample. This was also observed for PEF treated samples at an energy input of 256kJ/kg (37.0%, 15.6%, 29.4%, 26.5%, 23.5% for phenolics, anthocyanins, and antioxidant capacity using TEAC, ORAC and DPPH method, respectively). Consequently, pulsed electric technologies (HVED and PEF) show good prospects for enhanced bioaccessibility of compounds with putative health benefit.
采用体外模型比较了经脉冲电场（PEF）、高压放电（HVED）和超声（USN）技术处理的果葡糖浆-甜菊糖混合物在两种等效能量输入（32-256kJ/kg）下生物活性化合物的生物利用度和抗氧化能力。肠道消化后未检测到抗坏血酸，而 HVED、PEF 和 USN 处理增加了总类胡萝卜素的生物利用度。与未处理的样品相比，在 32kJ/kg 的能量输入下，HVED 处理提高了酚类化合物（34.2%）、花青素（31.0%）和抗氧化能力（TEAC、ORAC 和 DPPH 测定法分别为 35.8%、29.1%和 31.9%）的生物利用度。在 256kJ/kg 的能量输入下，PEF 处理的样品也观察到了同样的效果（酚类、花青素和抗氧化能力的 TEAC、ORAC 和 DPPH 测定法分别为 37.0%、15.6%、29.4%、26.5%、23.5%）。因此，脉冲电场技术（HVED 和 PEF）显示出提高具有潜在健康益处的化合物生物利用度的良好前景。
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