Yang Sung-Yong, Lee Sanghoon, Pyo Min Cheol, Jeon Hyeonjin, Kim Yoonsook, Lee Kwang-Won
Department of Biotechnology, College of Life Sciences and Biotechnology, Korea University, Seoul 02841, South Korea.
Korea Food Research Institute, Seongnam-si, Gyeongggi-do 13539, South Korea.
Food Chem. 2017 Apr 15;221:1979-1988. doi: 10.1016/j.foodchem.2016.11.145. Epub 2016 Nov 29.
High amounts of waste products generated from fish-processing need to be disposed of despite their potential nutritional value. A variety of methods, such as enzymatic hydrolysis, have been developed for these byproducts. In the current study, we investigated the physicochemical, biological and antioxidative properties of fish protein hydrolysates (FPH) conjugated with ribose through the Maillard reaction. These glycated conjugates of FPH (GFPH) had more viscous rheological properties than FPH and exhibited higher heat, emulsification and foaming stability. They also protected liver HepG2 cells against t-BHP-induced oxidative stress with enhanced glutathione synthesis in vitro. Furthermore, it was shown that GFPH induced upregulation of phase II enzyme expression, such as that of HO-1 and γ-GCL, via nuclear translocation of Nrf2 and phosphorylation of ERK. Taken together, these results demonstrate the potential of GFPH for use as a functional food ingredient with improved rheological and antioxidative properties.
尽管鱼类加工产生的大量废弃物具有潜在的营养价值，但仍需进行处理。针对这些副产品，已经开发了多种方法，如酶水解法。在本研究中，我们通过美拉德反应研究了与核糖结合的鱼蛋白水解物（FPH）的物理化学、生物学和抗氧化特性。这些FPH的糖化共轭物（GFPH）比FPH具有更粘稠的流变学特性，并且表现出更高的热稳定性、乳化稳定性和泡沫稳定性。它们还能在体外增强谷胱甘肽合成，保护肝癌HepG2细胞免受叔丁基过氧化氢（t-BHP）诱导的氧化应激。此外，研究表明，GFPH通过Nrf2核转位和ERK磷酸化诱导II期酶表达上调，如HO-1和γ-GCL的表达上调。综上所述，这些结果证明了GFPH作为一种具有改善流变学和抗氧化特性的功能性食品成分的潜力。
