壳聚糖生物材料调控形态发生素时空动力学以促进泪腺结构形成的数据。
Data supporting regulating temporospatial dynamics of morphogen for structure formation of the lacrimal gland by chitosan biomaterials.
作者信息
Hsiao Ya-Chuan, Yang Tsung-Lin
机构信息
Department of Otolaryngology, National Taiwan University Hospital and College of Medicine, Taipei, Taiwan, ROC; Department of Ophthalmology, Zhongxing Branch, Taipei City Hospital, Taipei, Taiwan, ROC; Department of Ophthalmology, College of Medicine, National Yang-Ming University, Taipei, Taiwan, ROC.
Department of Otolaryngology, National Taiwan University Hospital and College of Medicine, Taipei, Taiwan, ROC; Research Center for Developmental Biology and Regenerative Medicine, National Taiwan University, Taipei, Taiwan, ROC; Graduate Institute of Clinical Medicine, College of Medicine, National Taiwan University, Taipei 10002, Taiwan, ROC.
出版信息
Data Brief. 2016 Nov 19;10:108-115. doi: 10.1016/j.dib.2016.11.042. eCollection 2017 Feb.
The lacrimal gland is responsible for tear synthesis and secretion, and is derived from the epithelia of ocular surface and generated by branching morphogenesis. The dataset presented in this article is to support the research results of the effect of chitosan biomaterials on facilitating the structure formation of the lacrimal gland by regulating temporospatial dynamics of morphogen. The embryonic lacrimal gland explants were used as the standard experimental model for investigating lacrimal gland branching morphogenesis. Chitosan biomaterials promoted lacrimal gland branching with a dose-dependent effect. It helped binding of hepatocyte growth factor (HGF) related molecules in the epithelial-mesenchymal boundary of emerging epithelial branches. When mitogen-activated protein kinase (MAPK) or protein kinase B (Akt/PKB) inhibitors applied, the chitosan effects reduced. Nonetheless, the ratios of MAPK and Akt/PKB phosphorylation were still greater in the chitosan group than the control. The data demonstrated here confirm the essential role of HGF-signaling in chitosan-promoted structure formation of the lacrimal gland.
泪腺负责泪液的合成与分泌，它起源于眼表上皮，并通过分支形态发生形成。本文呈现的数据集旨在支持壳聚糖生物材料通过调节形态发生素的时空动态促进泪腺结构形成这一研究结果。胚胎泪腺外植体被用作研究泪腺分支形态发生的标准实验模型。壳聚糖生物材料以剂量依赖的方式促进泪腺分支。它有助于肝细胞生长因子（HGF）相关分子在新生上皮分支的上皮-间充质边界处结合。当应用丝裂原活化蛋白激酶（MAPK）或蛋白激酶B（Akt/PKB）抑制剂时，壳聚糖的作用减弱。尽管如此，壳聚糖组中MAPK和Akt/PKB的磷酸化比率仍高于对照组。此处展示的数据证实了HGF信号在壳聚糖促进泪腺结构形成中的关键作用。
Zotero 插件