巴西北部里奥格兰德州莫索罗非技术化养殖场猪群中猪支原体感染的高发生率。

High occurrence of Mycoplasma suis infection in swine herds from non-technified farms in Mossoró, state of Rio Grande do Norte, Northeastern Brazil.

作者信息

Toledo Mariana Aparecida, Leite Alexandro Iris, Gonçalves Luiz Ricardo, Sousa Keyla Carstens Marques de, Amaral Renan Bressianini do, Silva Glaucenyra Cecília Pinheiro da, Machado Rosangela Zacarias, André Marcos Rogério

机构信息

Faculdade de Ciências Agrárias e Veterinárias, Universidade Estadual Paulista "Júlio de Mesquita Filho" - UNESP, Jaboticabal, SP, Brasil.

Departamento de Ciência Animal, Universidade Federal Rural do Semi-Árido - UFERSA, Mossoró, RN, Brasil.

出版信息

Rev Bras Parasitol Vet. 2016 Oct-Dec;25(4):414-417. doi: 10.1590/S1984-29612016084. Epub 2016 Dec 8.

DOI:10.1590/S1984-29612016084

PMID:27982298

Abstract

Mycoplasma suis, the etiological agent of swine hemoplasmosis, has been neglected in swine herds around the world. Swine hemoplasmosis is frequently associated with hemolytic anemia, disgalacty, infertility and immunosuppression, and it results in significant economic losses. This study investigates the occurrence of M. suis in non-technified swine herds in the northeastern region of Brazil using quantitative PCR (qPCR) based on the 16S rRNA gene. Between March and August 2013, blood samples from 147 swine were collected during slaughter in the city of Mossoró, state of Rio Grande do Norte, northeastern Brazil. One hundred and twelve samples (76.19%) were positive for M. suis by qPCR assays. The range of Cqs and quantification (copies of a M. suis-16S rRNA gene fragment/µL) was 20.86-37.89 and 1.64×101-6.64×107, respectively. One can conclude that M. suis infection have high occurrence (76,19%) in non-technified swine-rearing systems in Mossoró in the state of Rio Grande do Norte, Brazil.

摘要

猪支原体，即猪溶血性贫血症的病原体，在世界各地的猪群中一直被忽视。猪溶血性贫血症常与溶血性贫血、泌乳障碍、不育和免疫抑制有关，并会导致重大经济损失。本研究基于16S rRNA基因，使用定量PCR（qPCR）技术调查了巴西东北部非技术化养猪场中猪支原体的感染情况。2013年3月至8月期间，在巴西东北部北里奥格兰德州莫索罗市屠宰场采集了147头猪的血样。通过qPCR检测，112份样本（76.19%）猪支原体呈阳性。Cq值范围和定量结果（猪支原体16S rRNA基因片段拷贝数/微升）分别为20.86 - 37.89和1.64×101 - 6.64×107。可以得出结论，在巴西北里奥格兰德州莫索罗市的非技术化养猪系统中，猪支原体感染发生率很高（76.19%）。