急性髓系白血病中MN1和PTEN基因的表达
MN1 and PTEN gene expression in acute myeloid leukemia.
作者信息
Zayed Rania A, Eltaweel Maha A, Botros Shahira K A, Zaki Mohamed A
机构信息
Clinical and Chemical Pathology Department, Faculty of Medicine, Cairo University, Giza, Egypt.
Clinical and Chemical Pathology Department, National Cancer Institute, Cairo University, Giza, Egypt.
出版信息
Cancer Biomark. 2017;18(2):177-182. doi: 10.3233/CBM-160235.
OBJECTIVE
Multiple genetic alterations with prognostic significance have been discovered in acute myeloid leukemia (AML). We studied the expression level of two genes, Meningioma1 (MN1) and Phosphatase and Tensin homolog (PTEN) to determine their expression in AML patients and their role as prognostic markers.
METHODS
The study included 50 cytogenetic normal de novo AML cases and 10 controls, Their level was detected by Real time Reverse Transcription-Polymerase Chain Reaction.
RESULT
Relative mRNA expression of MN1 was significantly higher (p value < 0.001) and PTEN expression was significantly lower (p value = 0.002). No correlation was found between neither MN1 nor PTEN mRNA expression and overall survival (p value = 0.212 and 0.310) respectively.
CONCLUSION
Although our study suggests a role for MN1 gene and PTEN genes in AML, we could not recommend their use as routine diagnostic and prognostic markers for AML in Egyptian population.
目的
在急性髓系白血病（AML）中已发现多种具有预后意义的基因改变。我们研究了脑膜瘤1（MN1）和磷酸酶及张力蛋白同源物（PTEN）这两个基因的表达水平，以确定它们在AML患者中的表达情况及其作为预后标志物的作用。
方法
该研究纳入了50例细胞遗传学正常的初发AML病例和10例对照，通过实时逆转录-聚合酶链反应检测它们的水平。
结果
MN1的相对mRNA表达显著更高（p值<0.001），而PTEN表达显著更低（p值=0.002）。MN1和PTEN的mRNA表达与总生存期之间均未发现相关性（p值分别为0.212和0.310）。
结论
尽管我们的研究提示MN1基因和PTEN基因在AML中发挥作用，但我们不建议将它们用作埃及人群AML的常规诊断和预后标志物。
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