Grinnell F, Minter D
Proc Natl Acad Sci U S A. 1978 Sep;75(9):4408-12. doi: 10.1073/pnas.75.9.4408.
Studies have been carried out to determine the effects of cold-insoluble globulin (CIG) on the attachment and spreading of baby hamster kidney cells on various collagen substrata. Cell attachment to native collagen substrata occurred in the absence of CIG just as fast as attachment to dried collagen or gelatin substrata occurred in the presence of CIG. On the other hand, cell attachment to dried collagen or gelatin was markedly reduced in the absence of CIG. Cell spreading also occurred on native collagen in the absence of CIG; however, CIG was absolutely required for cell spreading to occur on dried collagen or gelatin. Finally, anti-CIG antiserum or lactoperoxidase treatment inhibited cell spreading on CIG-coated substrata but not on native collagen substrata. The data are discussed in terms of the interaction of fibroblasts with collagen in situ.
已经开展了多项研究，以确定冷不溶性球蛋白（CIG）对幼仓鼠肾细胞在各种胶原蛋白基质上的附着和铺展的影响。在没有CIG的情况下，细胞附着于天然胶原蛋白基质的速度与在有CIG存在时细胞附着于干燥胶原蛋白或明胶基质的速度一样快。另一方面，在没有CIG的情况下，细胞对干燥胶原蛋白或明胶的附着明显减少。在没有CIG的情况下，细胞也能在天然胶原蛋白上铺展；然而，细胞在干燥胶原蛋白或明胶上发生铺展则绝对需要CIG。最后，抗CIG抗血清或乳过氧化物酶处理抑制了细胞在CIG包被的基质上的铺展，但不抑制在天然胶原蛋白基质上的铺展。根据成纤维细胞与原位胶原蛋白的相互作用对这些数据进行了讨论。
