Ling Jie, Zhang Wenzhong, Xu Bin, Qiu Wei, Wang Yongbing
Department of General Surgery, Pudong New Area People's Hospital, Shanghai 201200, China.
Zhonghua Wei Chang Wai Ke Za Zhi. 2016 Dec 25;19(12):1365-1369.
To explore the efficacy and the mechanism of Bisacodyl in treating slow transit constipation model rats.
A total of 30 healthy rats were enrolled. Twenty rats received intragastric diphenoxylate to develop slow transit constipation (STC) model, and 10 untreated rats were set as blank control. STC rats were subdivided into two groups: STC Bisacodyl group (fed with Bisacodyl) and STC control group (common feed). Body weight, number and dry weight of faeces, and intestinal transit time were compared among 3 groups. Interstitial cells of Cajal(ICC) and c-Kit protein expression were measured by immunohistochemical staining. Restults Compared to blank control rats, at 100-day of receiving intragastric diphenoxylate, above 20 rats presented the decrease of body weight and feces number, the increase of dry weight of faeces, and the delay of intestinal transit time, indicating the successful establishment of STC rat model. One month after feeding, compared to STC control group, STC Bisacodyl grap had an increased feces number[(36.6±6.8) pill/day vs. (26.8±6.0) pill/day], decreased dry weight of feces [(150.6±10.5) mg/pill vs. (171.6±16.3) mg/pill] and shortened intestinal transit time [(416.9±50.6) minutes vs. (495.3±66.8) minutes], and the differences were statistically significant(all P<0.05). Dissolution of ICC basement membrane, damage of connection between ICC and surrounding cells, and atrophy of ICC nucleus structure were found in STC control rats. ICC (8.20±1.92 per field] and c-Kit expression (12.68%±2.59% ) in STC control rats were significantly lower than those in blank control rats(36.00±6.25 per field and 71.50 %±8.27%) (P=0.000). Compared to STC control group, the connection between ICC and surrounding cells enhanced obviously, ICC (18.80±3.70 per field) and c-Kit expression (45.91%±6.80%) were significantly higher in STC Bisacodyl group (all P=0.000).
Bisacodyl treatment can relieve STC symptoms, which may be associated with increased ICC number and c-Kit protein expression.
探讨比沙可啶治疗慢传输型便秘模型大鼠的疗效及机制。
选取30只健康大鼠。20只大鼠经胃内给予地芬诺酯建立慢传输型便秘（STC）模型，10只未处理的大鼠作为空白对照。将STC大鼠分为两组：STC比沙可啶组（给予比沙可啶）和STC对照组（普通饲料）。比较三组大鼠的体重、粪便数量和干重以及肠道传输时间。采用免疫组织化学染色法检测Cajal间质细胞（ICC）和c-Kit蛋白表达。结果与空白对照大鼠相比，给予胃内地芬诺酯100天时，20只以上大鼠出现体重下降、粪便数量减少、粪便干重增加以及肠道传输时间延长，表明STC大鼠模型建立成功。喂养1个月后，与STC对照组相比，STC比沙可啶组粪便数量增加[（36.6±6.8）粒/天 vs.（26.8±6.0）粒/天]，粪便干重降低[（150.6±10.5）mg/粒 vs.（171.6±16.3）mg/粒]，肠道传输时间缩短[（416.9±50.6）分钟 vs.（495.3±66.8）分钟]，差异均有统计学意义（均P<0.05）。STC对照组大鼠可见ICC基底膜溶解、ICC与周围细胞连接破坏以及ICC核结构萎缩。STC对照组大鼠的ICC（每视野8.20±1.92个）和c-Kit表达（12.68%±2.59%）明显低于空白对照组大鼠（每视野36.00±6.25个和71.50%±8.27%）（P=0.000）。与STC对照组相比，STC比沙可啶组ICC与周围细胞连接明显增强，ICC（每视野18.80±3.70个）和c-Kit表达（45.91%±6.80%）明显升高（均P=0.000）。
比沙可啶治疗可缓解STC症状，其机制可能与增加ICC数量和c-Kit蛋白表达有关。
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