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一种预测的阳离子转运蛋白BPSS1228参与了类鼻疽杆菌在人肺上皮细胞系(A549)中的细胞内行为。

A predicted cation transporter protein, BPSS1228, is involved in intracellular behaviour of Burkholderia pseudomallei in a human lung epithelial cell line (A549).

作者信息

Techawiwattanaboon Teerasit, Chareonsudjai Sorujsiri

机构信息

Department of Microbiology, Faculty of Medicine, Melioidosis Research Center and Biofilm Research Group, Khon Kaen University, Khon Kaen, Thailand.

Department of Microbiology, Faculty of Medicine, Melioidosis Research Center and Biofilm Research Group, Khon Kaen University, Khon Kaen, Thailand

出版信息

FEMS Microbiol Lett. 2016 Dec;363(23). doi: 10.1093/femsle/fnw259. Epub 2016 Nov 13.

Abstract

Burkholderia pseudomallei causes melioidosis, a potentially fatal infectious disease in tropical and subtropical countries worldwide. The intracellular behaviour of this pathogen in host cells has been reported to impact the severity of melioidosis, including the development of septicaemia, a consequence of pneumonia melioidosis. We previously identified a predicted cation transporter protein, BPSS1228, that participates in the transitional stage of this intracellular pathogen. For further analysis, in this study B. pseudomallei bpss1228 mutant and complemented strains were constructed and bacterial infectivity on human lung epithelial cells, A549, investigated in vitro Burkholderia pseudomallei bpss1228 mutant showed impaired bacterial adhesion and invasion into A549 cells compared with wild-type strain, while the deficient phenotypes were restored to wild-type levels by the complemented strain. Additionally, the inactivation of bpss1228 in the mutant strain affected flagella-based swimming on a semi-solid surface and resistance to acid stresses simulating intracellular environments. These observations of BPSS1228 relating to B. pseudomallei infection strategies shed a new light on its association with intracellular B. pseudomallei during the interaction with host cells.

摘要

类鼻疽伯克霍尔德菌可引发类鼻疽,这是一种在全球热带和亚热带国家具有潜在致命性的传染病。据报道,这种病原体在宿主细胞内的行为会影响类鼻疽的严重程度,包括败血症的发生,而败血症是类鼻疽肺炎的一个后果。我们之前鉴定出一种预测的阳离子转运蛋白BPSS1228,它参与了这种细胞内病原体的过渡阶段。为了进一步分析,在本研究中构建了类鼻疽伯克霍尔德菌bpss1228突变体和互补菌株,并在体外研究了它们对人肺上皮细胞A549的细菌感染性。与野生型菌株相比,类鼻疽伯克霍尔德菌bpss1228突变体对A549细胞的黏附和侵袭能力受损,而互补菌株将这些缺陷表型恢复到了野生型水平。此外,突变菌株中bpss1228的失活影响了其在半固体表面基于鞭毛的游动以及对模拟细胞内环境的酸应激的抗性。这些关于BPSS1228与类鼻疽伯克霍尔德菌感染策略的观察结果为其在与宿主细胞相互作用期间与细胞内类鼻疽伯克霍尔德菌的关联提供了新的线索。

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