Department of Biophysics, Faculty of Sciences, VU University Amsterdam , De Boelelaan 1081, 1081 HV Amsterdam, The Netherlands.
Faculty of Science, Ibaraki University , Mito, Ibaraki 310-8512, Japan.
J Am Chem Soc. 2017 Jan 18;139(2):591-594. doi: 10.1021/jacs.6b11017. Epub 2017 Jan 3.
The purple bacterial core light harvesting antenna-reaction center (LH1-RC) complex is the simplest system able to achieve the entire primary function of photosynthesis. During the past decade, a variety of photosynthetic proteins were studied by a powerful technique, two-dimensional electronic spectroscopy (2DES). However, little attention has been paid to LH1-RC, although its reversible uphill energy transfer, trapping, and backward detrapping processes, represent a crucial step in the early photosynthetic reaction dynamics. Thus, in this work, we employed 2DES to study two LH1-RC complexes of Thermochromatium (Tch.) tepidum. By direct observation of detrapping, the complex reversible process was clearly identified and an overall scheme of the excitation evolution in LH1-RC was obtained.
紫细菌核心光捕获天线-反应中心（LH1-RC）复合物是能够实现光合作用全部初级功能的最简单系统。在过去的十年中，通过一种强大的技术——二维电子光谱（2DES）——研究了多种光合蛋白。然而，尽管 LH1-RC 的可逆 uphill 能量转移、捕获和反向解捕获过程代表了早期光合作用反应动力学中的一个关键步骤，但对其关注甚少。因此，在这项工作中，我们采用 2DES 研究了 Thermochromatium（Tch.）tepidum 的两个 LH1-RC 复合物。通过直接观察解捕获，可以清楚地识别出复合物的可逆过程，并获得 LH1-RC 中激发演化的总体方案。
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