Chen Junren, Shafi Mohammad, Guo Jia, Wang Ying, Wu Jiasen, Ye Zhengqian, He Lizhi, Liu Dan
Zhejiang A & F University, Key Laboratory of Soil Contamination Bioremediation of Zhejiang Province, The Key Nurturing Station for the State Key Laboratory of Subtropical Silviculture, Lin'an, Zhejiang 311300, PR China.
Zhejiang A & F University, Key Laboratory of Soil Contamination Bioremediation of Zhejiang Province, The Key Nurturing Station for the State Key Laboratory of Subtropical Silviculture, Lin'an, Zhejiang 311300, PR China; Zhejiang Resource Development Group Co., Ltd., 311300, PR China.
Ecotoxicol Environ Saf. 2017 Apr;138:71-77. doi: 10.1016/j.ecoenv.2016.12.020. Epub 2016 Dec 22.
The antioxidation system and accumulation ability of Moso bamboo (Phyllostachys pubescens), which is a valuable remediation material with large biomass and rapid growth rate were studied in hydroponics and pot experiments. In hydroponics experiment, TBARS concentrations and SOD activities decreased with increase of Pb treatments. The activities of POD boost up with elevated Pb treatments, and reached peak level with application of 400μM Pb. Proline concentrations reduced with application of 20μM Pb and then enhanced consistently with application of 100 and 400μM Pb. The biomass of Moso bamboo improved with increase of Pb treatments upto 400mgkg, and then decreased with application of each additional increment of Pb in pot experiment. Application of 800mgkg Pb showed significant increase of photosynthetic pigments, however, non significant variation was observed for other treatments. The Pb concentration in roots, stems and leaves attained 523mgkg, 303mgkg and 222mgkg respectively with application of 1600mgkg Pb compared with control. Analysis of TEM-EDX revealed that Pb in cell was mostly concentrated in cytoplasm then in cell wall and followed by vacuole. It is concluded that Moso bamboo may be potential remediation species for phytoremediation in low Pb contaminated soils.
毛竹（Phyllostachys pubescens）是一种具有巨大生物量和快速生长速率的珍贵修复材料，通过水培和盆栽试验研究了其抗氧化系统和积累能力。在水培试验中，丙二醛（TBARS）浓度和超氧化物歧化酶（SOD）活性随铅处理浓度的增加而降低。过氧化物酶（POD）活性随铅处理浓度的升高而增强，在施加400μM铅时达到峰值。脯氨酸浓度在施加20μM铅时降低，然后在施加100μM和400μM铅时持续增加。在盆栽试验中，毛竹生物量随铅处理浓度增加至400mg/kg而增加，然后随每增加一次铅浓度而降低。施加800mg/kg铅时光合色素显著增加，然而其他处理未观察到显著变化。与对照相比，施加1600mg/kg铅时，根、茎和叶中的铅浓度分别达到523mg/kg、303mg/kg和222mg/kg。透射电子显微镜-能谱分析（TEM-EDX）表明，细胞中的铅大多集中在细胞质中，然后是细胞壁，其次是液泡。得出结论，毛竹可能是低铅污染土壤植物修复的潜在修复物种。
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