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海洋学中的生物发光。

Bioluminescence in oceanology.

作者信息

Gitelson I I, Levin L A

机构信息

Institute of Biophysics, USSR Academy of Sciences, Krasnoyarsk.

出版信息

J Biolumin Chemilumin. 1989 Jul;4(1):555-62. doi: 10.1002/bio.1170040173.

Abstract

For analytical purposes bioluminescence can be used in three main ways: 1. luminescence measurement of bioluminescent system components isolated in vitro; 2. determination of luminous organisms' reaction to the in vivo test-action; 3. measurement of bioluminescence in marine ecological systems. The majority of the reports of this Symposium are dealing with the first two topics. The aim of our presentation is to draw attention to the third one. The possibilities of bioluminescent analysis are wider than its traditional scheme of applications in the laboratory, when the emitting system is withdrawn from a native source and is placed in a cuvette of the light measuring device. The reverse scheme is also possible, i.e. the device can be introduced into light emitting system such as a marine biocenosis--the community of the sea inhabitants--where we obtain a highly sensitive and rapid means of gaining the information on the vital activity of marine ecosystems, i.e. their spatial structure, rhythms, man's influence upon them, etc. The present communication will consider the possibilities of this form of bioluminescent analysis.

摘要

出于分析目的,生物发光主要有三种应用方式:1. 对体外分离的生物发光系统组件进行发光测量;2. 测定发光生物体对体内测试作用的反应;3. 测量海洋生态系统中的生物发光。本次研讨会的大多数报告都涉及前两个主题。我们演讲的目的是让大家关注第三个主题。生物发光分析的可能性比其在实验室中的传统应用方案更广泛,传统方案是将发光系统从天然来源取出并置于光测量设备的比色皿中。相反的方案也是可行的,即可以将设备引入发光系统中,例如海洋生物群落——海洋生物的群落——在那里我们获得了一种高度灵敏且快速的手段,以获取有关海洋生态系统生命活动的信息,即它们的空间结构、节律、人类对它们的影响等。本报告将探讨这种生物发光分析形式的可能性。

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