利用圆二色性研究蛙皮抗菌肽2和天蚕素A与大肠杆菌细胞的相互作用。
Studying the Interaction of Magainin 2 and Cecropin A with E. coli Bacterial Cells Using Circular Dichroism.
作者信息
Avitabile Concetta, D'Andrea Luca Domenico, Romanelli Alessandra
机构信息
Institute of Biostructure and Bioimaging CNR, via Mezzocannnone 16, 80134, Naples, Italy.
Department of Pharmacy, University of Naples "Federico II", via Mezzocannone 16, Naples, 80134, Italy.
出版信息
Methods Mol Biol. 2017;1548:247-253. doi: 10.1007/978-1-4939-6737-7_17.
The potential of antimicrobial peptides (AMPs) as an effective therapeutic alternative to classic and current antibiotics has encouraged studies to understand how they interact with the bacterial membrane. Here we describe how to detect, by circular dichroism (CD), the secondary structures of two antimicrobial peptides, magainin 2 and cecropin A, in the presence of E. coli bacterial cells.
抗菌肽（AMPs）作为经典抗生素和现有抗生素的有效治疗替代物的潜力，促使人们开展研究以了解它们如何与细菌膜相互作用。在此，我们描述了如何通过圆二色性（CD）检测两种抗菌肽——蛙皮素2和天蚕素A在大肠杆菌细胞存在时的二级结构。
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