抗C1q/TNF相关蛋白4单克隆抗体的制备与鉴定
Preparation and Identification of Monoclonal Antibody Against C1q/TNF-Related Protein 4.
作者信息
Huang He, Wu Xiaotong, Cao Lulu, Wang Lu
机构信息
Department of Immunology, School of Basic Medical Sciences, Peking University Health Science Center , Beijing, China .
出版信息
Monoclon Antib Immunodiagn Immunother. 2016 Dec;35(6):280-284. doi: 10.1089/mab.2016.0027.
CTRP4 (C1q/TNF-related protein 4) is a secreted cytokine homologous to adiponectin, which plays an important role in immunity and metabolism. This study was designed to generate CTRP4 monoclonal antibodies (MAbs). Splenocytes extracted from mice immunized with prokaryotic protein were fused with myeloma cells Sp2/0 to generate hybridoma cells. Three hybridoma strains (16, 33, and 35) were chosen and their MAbs were purified. The specificity and affinity were identified by Western blot and enzyme-linked immunosorbent assay, whereas CTRP4 endogenous expression was identified by immunofluorescence staining. These MAbs could be useful tools for basic research and potential clinical application.
CTRP4（C1q/TNF相关蛋白4）是一种与脂联素同源的分泌细胞因子，在免疫和代谢中起重要作用。本研究旨在制备CTRP4单克隆抗体（MAb）。用原核蛋白免疫小鼠提取的脾细胞与骨髓瘤细胞Sp2/0融合以产生杂交瘤细胞。选择了三株杂交瘤菌株（16、33和35）并纯化了它们的单克隆抗体。通过蛋白质免疫印迹法和酶联免疫吸附测定法鉴定特异性和亲和力，而通过免疫荧光染色鉴定CTRP4内源性表达。这些单克隆抗体可能是基础研究和潜在临床应用的有用工具。
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