Braggio Morucchio G, Cornara L, Dellachà E
Boll Soc Ital Biol Sper. 1989 Aug;65(8):695-701.
Pollen grains from samples of Camellia japonica living in soil (A) and in greenhouse (B) were collected daily from just-opened anthers. The pollen was sown in various liquid media and incubated at 28 C. Higher germinative ability of A in comparison with B was observed and related to the relative humidity which is higher in soil than in greenhouse. This phenomenon recurs even if the composition of the culture medium is changed. However the composition of the medium greatly influences the percentage of germination of both A and B notwithstanding the environmental conditions. The pollen collected from anthers dehiscing in the first day of the anthesis has a high germinative ability which suddenly decreases in the ripe pollen of the following days.
每天从刚开放的花药中收集生长在土壤（A）和温室（B）中的山茶花样本的花粉粒。将花粉播种在各种液体培养基中，并在28℃下培养。观察到与B相比，A具有更高的萌发能力，这与土壤中相对湿度高于温室中的相对湿度有关。即使改变培养基的成分，这种现象也会再次出现。然而，尽管环境条件不同，培养基的成分对A和B的萌发率都有很大影响。在花期第一天开裂的花药中收集的花粉具有较高的萌发能力，而在接下来几天的成熟花粉中，萌发能力会突然下降。
