Abbo Olivier, Taurand Marion, Monsarrat Paul, Raymond Isabelle, Arnaud Emmanuelle, De Barros Sandra, Auriol Françoise, Galinier Philippe, Casteilla Louis, Planat-Benard Valerie
STROMALab, Université de Toulouse, Toulouse, France; Service de Chirurgie Pédiatrique, Hôpital des Enfants de Toulouse, Toulouse, France.
STROMALab, Université de Toulouse, Toulouse, France.
Cytotherapy. 2017 Mar;19(3):395-407. doi: 10.1016/j.jcyt.2016.11.012. Epub 2016 Dec 28.
Adipose-derived mesenchymalstromal cells (ASC) are currently tested in regenerative medicine to promote tissue reconstruction after injury. Regardingautologous purpose, the possible loss of therapeutic function and cell properties during aging have been questioned in adults. To date no reliable information is available concerning ASC from pediatric patients and a better knowledge is required for clinical applications.
Subcutaneous adipose tissue was collected from 27 donors (0-1 years old) and 50 donors (1-12 years old) and compared with adult ASC for in vitro characteristics. ASC were then tested in a mouse model of limb ischemia.
Cells from the stromal vascular fraction (SVF) and subsequent cultured ASC were prepared. Only a greater amount in SVF cell number and ASC proliferative rate were found. Cell phenotype, colony formingunit-fibroblast (CFU-F) content, immunomodulation effect and adipogenic, osteoblastic and angiogenic potentials were not significantly different. In vivo, pediatric ASC induced an increase in microangiographic score in a mouse model of limb ischemia, even though improvement in vascular density was not significantly correlated to limb rescue. Finally messengerRNA (mRNA) analysis using a microarray approach identified that only 305 genes were differentially expressed (217 down- and 88 up-regulated) in pediatric versus adult ASC, confirming that ASC from both age groups shared very close intrinsic properties.
This is the first study reporting a comparative analysis of ASC from a large number of donors and showing that their in vitro and in vivo properties were similar and maintained during aging.
脂肪来源的间充质基质细胞（ASC）目前正在再生医学中进行测试，以促进损伤后的组织重建。就自体应用而言，成人衰老过程中治疗功能和细胞特性可能丧失的问题受到了质疑。迄今为止，关于儿科患者ASC的可靠信息尚不可得，临床应用需要更深入的了解。
从27名0至1岁的供体和50名1至12岁的供体中收集皮下脂肪组织，并与成人ASC进行体外特性比较。然后在肢体缺血小鼠模型中对ASC进行测试。
制备了来自基质血管部分（SVF）的细胞以及随后培养的ASC。仅发现SVF细胞数量和ASC增殖率更高。细胞表型、集落形成单位-成纤维细胞（CFU-F）含量、免疫调节作用以及成脂、成骨和血管生成潜能无显著差异。在体内，儿科ASC在肢体缺血小鼠模型中诱导微血管造影评分增加，尽管血管密度的改善与肢体挽救无显著相关性。最后，使用微阵列方法进行的信使核糖核酸（mRNA）分析表明，儿科ASC与成人ASC相比，只有305个基因差异表达（217个下调和88个上调），证实两个年龄组的ASC具有非常相似的内在特性。
这是第一项对大量供体的ASC进行比较分析的研究，表明它们的体外和体内特性相似，且在衰老过程中保持不变。
