制备脑片以研究基本突触特性。

Preparing Brain Slices to Study Basic Synaptic Properties.

作者信息

Xu-Friedman Matthew A

机构信息

Department of Biological Sciences, University at Buffalo, State University of New York, Buffalo, New York 14260.

出版信息

Cold Spring Harb Protoc. 2017 Jan 3;2017(1):2017/1/pdb.prot093328. doi: 10.1101/pdb.prot093328.

DOI:10.1101/pdb.prot093328

PMID:28049780

Abstract

This protocol describes how to prepare brain slices for electrophysiology, with an emphasis on synaptic physiology in voltage clamp. This approach remains the gold standard for understanding the properties of individual neurons, as well as the connections between neurons.

摘要

本方案描述了如何制备用于电生理学研究的脑片，重点在于电压钳下的突触生理学。这种方法仍然是理解单个神经元特性以及神经元之间连接的金标准。