Xu Mengxue, Feng Chao, Wang Juan, Lang Xuqian, Xia Guixue, Yu Xiaoping, Ji Qiuxia, Cheng Xiaojie, Kong Ming, Liu Ya, Chen Xiguang
a College of Marine Life Science , Ocean University of China , Qingdao , China.
b College of Life Science , Linyi University , Linyi , China.
J Biomater Sci Polym Ed. 2017 Mar;28(4):380-393. doi: 10.1080/09205063.2016.1277624. Epub 2017 Jan 18.
In the present work, sodium alginate (ALG) was degraded by heterogeneous phase acid degradation. The molecular weight distribution of ALG after degradation was close to homogenization. Then the blood bio-compatibility of ALG with different molecular weights (ALG-0h 50,075, ALG-0.5h 20,680, ALG-2h 13,170 and ALG-96h 1170 kDa) was evaluated in vitro and vivo. The human umbilical vein endothelial cells were used to assess the cytotoxicity of ALGs, ALG-0.5h and ALG-2h exhibited greater increment in percentage of cell viability comparing with ALG-0h and ALG-96h. With increasing of molecular weight of ALG, the blood clotting time was shortened and the hemolysis rate was slightly decreased. The different degree aggregation of red blood cells (RBCs) was observed in the ALG with different molecular groups and ALG-0h caused a severe aggregation of RBCs. Hematology analysis in vivo behavior after intraperitoneal (i.p.) injection indicated ALG-0h could cause blood solidification. Above results provided a reference for molecular weight selection in different applications.
在本研究中，海藻酸钠（ALG）通过多相酸降解法进行降解。降解后ALG的分子量分布趋于均匀。然后对不同分子量的ALG（ALG-0h 50,075、ALG-0.5h 20,680、ALG-2h 13,170和ALG-96h 1170 kDa）进行了体内外血液生物相容性评价。用人脐静脉内皮细胞评估ALG的细胞毒性，与ALG-0h和ALG-96h相比，ALG-0.5h和ALG-2h的细胞活力百分比增幅更大。随着ALG分子量的增加，凝血时间缩短，溶血率略有降低。在不同分子基团的ALG中观察到红细胞（RBC）不同程度的聚集，ALG-0h导致RBC严重聚集。腹腔注射后体内血液学分析表明ALG-0h可导致血液凝固。上述结果为不同应用中分子量的选择提供了参考。
