反向微乳液体系中磷脂酶A1催化合成富含二十二碳六烯酸的磷脂酰胆碱
Phospholipase A1-Catalysed Synthesis of Docosahexaenoic Acid-Enriched Phosphatidylcholine in Reverse Micelles System.
作者信息
Chen Wuxi, Guo Wei, Gao Feng, Chen Limei, Chen Shulin, Li Demao
机构信息
Tianjin Key Laboratory for Industrial Biological Systems and Bioprocess Engineering, Tianjin Institute of Industrial Biotechnology, Chinese Academy of SciencesTianjin, No. 32, Xiqi Road, Tianjin Airport Economic Park, Tianjin, 300308, People's Republic of China.
出版信息
Appl Biochem Biotechnol. 2017 Jul;182(3):1037-1052. doi: 10.1007/s12010-016-2379-y. Epub 2017 Jan 7.
Phosphatidylcholine enriched with docosahexaenoic acid (DHA) was successfully produced by phospholipase A1-catalysed acidolysis. Reverse micelles were firstly selected as the reaction system to avoid the process of immobilization and to ensure the efficient catalysis of phospholipase A1. Parameters were optimized for a high incorporation of phosphatidylcholine enriched with DHA (DHA-PC). A response-surface design with four factors, including the water content, enzyme loading, pH and substrate-mass ratio were used to evaluate the influence of the major factors and to predict the optimal reaction conditions. The results indicated that the optimal reaction conditions for the production of DHA-PC were a water content of 0.4%, an enzyme loading of 40%, pH 6.92 and a substrate-mass ratio of 2.13. Under these optimized conditions, 20.90% of DHA content in DHA-PC was obtained.
通过磷脂酶A1催化的酸解反应成功制备了富含二十二碳六烯酸（DHA）的磷脂酰胆碱。首先选择反胶束作为反应体系，以避免固定化过程并确保磷脂酶A1的高效催化。对富含DHA的磷脂酰胆碱（DHA-PC）的高掺入量进行了参数优化。采用包含水含量、酶负载量、pH值和底物质量比四个因素的响应面设计来评估主要因素的影响并预测最佳反应条件。结果表明，制备DHA-PC的最佳反应条件为水含量0.4%、酶负载量40%、pH值6.92和底物质量比2.13。在这些优化条件下，DHA-PC中的DHA含量达到了20.90%。
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