Pak C C, Fidler I J
Department of Cell Biology, University of Texas, M.D. Anderson Cancer Center, Houston 77030.
Differentiation. 1989 Jul;41(1):49-55. doi: 10.1111/j.1432-0436.1989.tb00731.x.
The interaction between macrophages and differentiating cells was examined using murine erythroleukemia cells (MELC). Inflammatory macrophages activated with recombinant murine interferon-gamma (rMuIFN-gamma) and lipopolysaccharide (LPS) first specifically recognized and bound tumorigenic-undifferentiated MELC and then produced their lysis. MELC that were induced to differentiate by a 5-day treatment with 5 mM N,N'-hexamethylene-bis-acetamide (HMBA) accumulated hemoglobin (benzidine positive) and were not recognized by the macrophages. Qualitative examination by light and electron microscopy confirmed the specific nature of the macrophage-MELC interaction. Quantitative assessment showed that the binding was dependent on the temperature and divalent cations and independent of serum components. A 24-h treatment of MELC with HMBA resulted in decreased binding, prior to hemoglobin accumulation and commitment to differentiation. The lack of binding of nontumorigenic-differentiated cells by macrophages was not due to residual HMBA. It thus appears that macrophages can distinguish MELC at different stages of differentiation.
利用小鼠红白血病细胞（MELC）研究了巨噬细胞与分化细胞之间的相互作用。用重组小鼠干扰素 -γ（rMuIFN -γ）和脂多糖（LPS）激活的炎性巨噬细胞首先特异性识别并结合致瘤性未分化的MELC，然后使其裂解。经5 mM N，N'-六亚甲基双乙酰胺（HMBA）处理5天诱导分化的MELC积累了血红蛋白（联苯胺阳性），未被巨噬细胞识别。光镜和电镜的定性检查证实了巨噬细胞与MELC相互作用的特异性。定量评估表明，结合依赖于温度和二价阳离子，且与血清成分无关。在血红蛋白积累和开始分化之前，用HMBA对MELC进行24小时处理导致结合减少。巨噬细胞对非致瘤性分化细胞缺乏结合并非由于残留的HMBA。因此，巨噬细胞似乎能够区分处于不同分化阶段的MELC。
