Oyinbo Charles A, Igbigbi Patrick S, Avwioro Godwin O
Department of Human Anatomy, Faculty of Basic Medical Sciences, College of Health Sciences, Niger Delta University, Wilberforce Island, Bayelsa State, Nigeria
Department of Anatomy and Cell Biology, Faculty of Basic Medical Sciences, Delta State University, Abraka, Delta State, Nigeria
Folia Med (Plovdiv). 2016 Dec 1;58(4):241-249. doi: 10.1515/folmed-2016-0034.
Alcohol-induced cerebellar neurodegeneration is a neuroadaptation that is associated with chronic alcohol abuse. Conventional drugs have been largely unsatisfactory in preventing neurodegeneration. Yet, multimodal neuro-protective therapeutic agents have been hypothesised to have high therapeutic potential for the treatment of CNS conditions; there is yet a dilemma of how this would be achieved. Contrarily, medicinal botanicals are naturally multimodal in their mechanism of action.
The effect of L. owariensis was therefore assessed in alcohol-induced neurodegeneration of the cerebellar cortex in rats.
Two groups of rats were oro-gastrically fed thrice daily with 5 g/kg ethanol (25% w/v), and 5 g/kg ethanol (25% w/v) plus L. owariensis (100 mg/kg body weight) respectively in diluted nutritionally complete diet (50% v/v). A control group was correspondingly fed a nutritionally complete diet (50% v/v) made isocaloric with glucose. Cytoarchitectural study of the cerebellar cortex was examined with H&E. Immunocytochemical analysis was carried out with the use of monoclonal antibody anti-NF in order to detect alterations in the neuronal cytoskeleton.
After 4 days of binge alcohol treatment, we observed that L. owariensis supplementation significantly lowered the levels of histologic and biochemical indices of neurodegeneration. The level of neurodegeneration and cytoarchitecture distortion of the cerebellar cortex of rats exposed to ethanol was reduced by L. owariensis. Neurofilament-immunoreactivity (NF-IR) was evoked in the Purkinje cells of rats that received L. owariensis supplement.
L. owariensis attenuates alcohol-induced cerebellar degeneration in the rat by alleviating oxidative stress and alteration of NF protein expression in the Purkinje cells.
酒精性小脑神经变性是一种与慢性酒精滥用相关的神经适应性变化。传统药物在预防神经变性方面大多不尽人意。然而，多模式神经保护治疗剂被认为对中枢神经系统疾病具有很高的治疗潜力；但如何实现这一点仍存在困境。相反，药用植物在其作用机制上天然具有多模式性。
因此，评估了奥瓦里豆（L. owariensis）对大鼠酒精诱导的小脑皮质神经变性的影响。
两组大鼠每天经口灌胃三次，分别给予5 g/kg乙醇（25% w/v），以及5 g/kg乙醇（25% w/v）加奥瓦里豆（100 mg/kg体重），均用稀释的营养完全日粮（50% v/v）。相应地，对照组给予与葡萄糖等热量的营养完全日粮（50% v/v）。用苏木精和伊红（H&E）对小脑皮质进行细胞结构研究。使用抗神经丝单克隆抗体进行免疫细胞化学分析，以检测神经元细胞骨架的变化。
在暴饮酒精处理4天后，我们观察到补充奥瓦里豆显著降低了神经变性的组织学和生化指标水平。奥瓦里豆降低了暴露于乙醇的大鼠小脑皮质的神经变性水平和细胞结构扭曲。补充奥瓦里豆的大鼠浦肯野细胞中诱发了神经丝免疫反应性（NF-IR）。
奥瓦里豆通过减轻氧化应激和浦肯野细胞中NF蛋白表达的改变，减轻大鼠酒精诱导的小脑变性。
