Mršić Gordan, Ozretić Petar, Crnjac Josip, Merkaš Siniša, Račić Ivana, Rožić Sara, Sukser Viktorija, Popović Maja, Korolija Marina
Forensic Science Centre "Ivan Vučetić", Zagreb, Croatia.
Forensic Science Office, University of Zagreb, Zagreb, Croatia.
Mol Biol Rep. 2017 Feb;44(1):183-189. doi: 10.1007/s11033-017-4096-1. Epub 2017 Jan 9.
The aim of the study was to assess forensic pertinence of 12 short tandem repeats (STRs) on X-chromosome in south Croatia population. Investigator Argus X-12 kit was used to co-amplify 12 STR loci belonging to four linkage groups (LGs) on X-chromosome in 99 male and 98 female DNA samples of unrelated donors. PCR products were analyzed by capillary electrophoresis. Population genetic and forensic parameters were calculated by the Arlequin and POPTREE2 software, and an on-line tool available at ChrX-STR.org. Hardy-Weinberg equilibrium was confirmed for all X-STR markers in female samples. Biallelic patterns at DXS10079 locus were detected in four male samples. Polymorphism information content for the most (DXS10135) and the least (DXS8378) informative markers was 0.9212 and 0.6347, respectively. In both male and female samples, combined power of discrimination exceeded 0.999999999. As confirmed by linkage disequilibrium test, significant association of marker pair DXS10074-DXS10079 (P = 0.0004) within LG2 and marker pair DXS10101-DXS10103 (P = 0.0003) within LG3 was found only in male samples. Number of observed haplotypes in our sample pool amounted 3.01, 7.53, 5 and 3.25% of the number of possible haplotypes for LG1, LG2, LG3 and LG4, respectively. According to haplotype diversity value of 0.9981, LG1 was the most informative. In comparison of south Croatia with 26 world populations, pair-wise [Formula: see text] values increase in parallel with geographical distance. Overall statistical assessment confirmed suitability of Investigator Argus X-12 kit for forensic casework in both identification and familial testing in the population of south Croatia.
本研究的目的是评估克罗地亚南部人群中X染色体上12个短串联重复序列（STR）的法医相关性。使用Investigator Argus X-12试剂盒对99份男性和98份女性无关供体的DNA样本中属于X染色体上四个连锁群（LG）的12个STR基因座进行共扩增。PCR产物通过毛细管电泳进行分析。群体遗传和法医参数通过Arlequin和POPTREE2软件以及ChrX-STR.org上的在线工具进行计算。女性样本中所有X-STR标记均符合哈迪-温伯格平衡。在四个男性样本中检测到DXS10079基因座的双等位基因模式。信息含量最高（DXS10135）和最低（DXS8378）的标记的多态性信息含量分别为0.9212和0.6347。在男性和女性样本中，联合鉴别力均超过0.999999999。连锁不平衡检验证实，仅在男性样本中发现LG2内的标记对DXS10074-DXS10079（P = 0.0004）和LG3内的标记对DXS10101-DXS10103（P = 0.0003）存在显著关联。我们样本库中观察到的单倍型数量分别占LG1、LG2、LG3和LG4可能单倍型数量的3.01%、7.53%、5%和3.25%。根据单倍型多样性值0.9981，LG1信息含量最高。将克罗地亚南部与26个世界人群进行比较时，成对的[公式：见原文]值随地理距离平行增加。总体统计评估证实，Investigator Argus X-12试剂盒适用于克罗地亚南部人群法医鉴定和家系检测的实际案件工作。
