Ferroni Felix Martin, Tolmie Carmien, Smit Martha Sophia, Opperman Diederik Johannes
Department of Biotechnology, University of the Free State, 205 Nelson Mandela Drive, Bloemfontein, 9300, South Africa.
Chembiochem. 2017 Mar 16;18(6):515-517. doi: 10.1002/cbic.201600684. Epub 2017 Feb 22.
We investigated Baeyer-Villiger monooxygenase (BVMO)-mediated synthesis of alkyl formate esters, which are important flavor and fragrance products. A recombinant fungal BVMO from Aspergillus flavus was found to transform a selection of aliphatic aldehydes into alkyl formates with high regioselectivity. Near complete conversion of 10 mm octanal was achieved within 8 h with a regiomeric excess of ∼80 %. Substrate concentration was found to affect specific activity and regioselectivity of the BVMO, as well as the rate of product autohydrolysis to the primary alcohol. More than 80 % conversion of 50 mm octanal was reached after 72 h (TTN nearly 20 000). Biotransformation on a 200 mL scale under unoptimized conditions gave a space-time yield (STY) of 4.2 g L d (3.4 g L d extracted product).
我们研究了拜耳-维利格单加氧酶（BVMO）介导的甲酸烷基酯的合成，甲酸烷基酯是重要的香料和香精产品。发现来自黄曲霉的重组真菌BVMO能以高区域选择性将多种脂肪醛转化为甲酸烷基酯。在8小时内实现了10 mmol辛醛接近完全转化，区域异构体过量约80%。发现底物浓度会影响BVMO的比活性和区域选择性，以及产物自水解为伯醇的速率。72小时后50 mmol辛醛的转化率超过80%（总转化数接近20000）。在未优化条件下200 mL规模的生物转化时空产率（STY）为4.2 g L⁻¹ d⁻¹（提取产物为3.4 g L⁻¹ d⁻¹）。
