Beyer J D, Milani F X, Dutelle M J, Bradley R L
University of Wisconsin, Department of Food Science, Madison 53706.
J Assoc Off Anal Chem. 1989 Sep-Oct;72(5):746-8.
A method has been developed for quantification of cholesterol in fresh egg yolks, spray-dried egg yolks, fresh whole eggs, and spray-dried whole eggs. The method uses saponification followed by petroleum ether extraction of cholesterol. Separation of organic and aqueous layers is enhanced by sodium chloride. Petroleum ether extracts are dried under nitrogen and redissolved in chloroform-methanol (2 + 1) for injection into a gas chromatograph. Cholesterol is separated and quantitated on a high temperature capillary column coated with 5% diphenyl and 95% dimethyl polysilicone crosslinked gum. The method was compared with the current AOAC method 17.017-17.022, and results indicated no significant difference (alpha = 0.05). However, the proposed method allowed separation and analysis of 16 samples in 7 h while the current AOAC method allowed separation and analysis of only 4 samples in 9 h.
已开发出一种用于定量测定新鲜蛋黄、喷雾干燥蛋黄、新鲜全蛋和喷雾干燥全蛋中胆固醇的方法。该方法采用皂化，然后用石油醚萃取胆固醇。氯化钠可增强有机层和水层的分离。石油醚萃取物在氮气下干燥，再溶解于氯仿 - 甲醇（2 + 1）中，注入气相色谱仪。胆固醇在涂有5%二苯基和95%二甲基聚硅氧烷交联胶的高温毛细管柱上进行分离和定量。将该方法与现行的AOAC方法17.017 - 17.022进行比较，结果表明无显著差异（α = 0.05）。然而，所提出的方法能够在7小时内分离并分析16个样品，而现行的AOAC方法在9小时内仅能分离并分析4个样品。
