桑科15个多态性微卫星标记的开发。
Development of 15 polymorphic microsatellite markers for (Moraceae).
作者信息
Fu Rong-Hua, Li Yun-Xiang, Liu Mei, Quan Qiu-Mei
机构信息
Key Laboratory of Southwest China Wildlife Resources Conservation (Ministry of Education), China West Normal University, Nanchong, Sichuan 637002, People's Republic of China; Ecological Security and Protection Key Laboratory of Sichuan Province, Mianyang Normal University, Sichuan 621000, People's Republic of China.
Key Laboratory of Southwest China Wildlife Resources Conservation (Ministry of Education), China West Normal University, Nanchong, Sichuan 637002, People's Republic of China.
出版信息
Appl Plant Sci. 2017 Jan 6;5(1). doi: 10.3732/apps.1600101. eCollection 2017 Jan.
PREMISE OF THE STUDY
(Moraceae) is distributed widely in South and Southeast Asia, Melanesia, and northern Australia, and it is also cultivated outside its original northern range limit in southwestern China. Therefore, the species is well suited to explore the mechanism of range limits of species. However, little is known about its genetic background.
METHODS AND RESULTS
Fifteen polymorphic microsatellite markers were developed using the biotin-streptavidin capture method. Polymorphism was tested in 85 individuals sampled from three populations. The number of alleles ranged from three to 17. The observed and expected heterozygosity of each population varied from 0.0667 to 0.9286 and 0.0650 to 0.8890, respectively. Cross-species amplification was also carried out in eight other species.
CONCLUSIONS
These 15 markers will be valuable for studying the genetic variation and population structure of and related species.
研究前提
（桑科）广泛分布于南亚和东南亚、美拉尼西亚以及澳大利亚北部，在中国西南部其原生分布范围的北界之外也有栽培。因此，该物种非常适合用于探究物种分布范围界限的机制。然而，对其遗传背景了解甚少。
方法与结果
采用生物素-链霉亲和素捕获法开发了15个多态性微卫星标记。在从三个种群中采集的85个个体中测试了多态性。等位基因数量从3个到17个不等。每个种群的观察杂合度和期望杂合度分别在0.0667至0.9286和0.0650至0.8890之间变化。还在其他八个物种中进行了跨物种扩增。
结论
这15个标记对于研究该物种及其相关物种的遗传变异和种群结构将具有重要价值。
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