Luu Trang H, Michel Catherine, Bard Jean-Marie, Dravet François, Nazih Hassan, Bobin-Dubigeon Christine
a UNAM Université de Nantes, Faculté de Pharmacie, EA 2160 MMS-Institut Universitaire Mer et Littoral FR3473 CNRS, Centre de Recherche en Nutrition Humaine Ouest (CRNH Ouest) , Nantes , France.
b UMR 1280 Physiologie des adaptations nutritionnelles , Nantes , France.
Nutr Cancer. 2017 Feb-Mar;69(2):267-275. doi: 10.1080/01635581.2017.1263750. Epub 2017 Jan 17.
Improving knowledge about breast cancer etiology is crucial in order to propose prevention strategies for this pathology. Gut microbiota is involved in numerous physiopathological situations including cancers. Although its potential involvement in breast cancer through the alteration of the enterohepatic circulation of estrogens and/or the metabolism of phytoestrogens has been discussed for some time, it remains to be demonstrated. The present study seeks to strengthen this hypothesis by identifying possible links between the fecal microbiota composition and clinical characteristics in breast cancer patients. Bacterial DNA was extracted from the feces of 31 patients with early-stage breast cancer and amplified by real-time polymerase chain reaction (qPCR), targeting 16S rRNA sequences specific to bacterial groups, and then analyzed in relation to clinical characteristics. The absolute numbers of total bacteria and of three bacterial groups (Firmicutes, Faecalibacterium prausnitzii, and Blautia) differed significantly according to the patient's body mass index. The percentage and the absolute numbers of certain bacterial groups, namely C. coccoides, F. prausnitzii, and Blautia, differed significantly according to the clinical stages and the histoprognostic grades. Our study highlighted that intestinal microbiota composition in these patients differs according to clinical characteristics and BMI. Further studies are required to clarify the link between breast cancer and intestinal microbiota.
为了提出针对这种疾病的预防策略，提高对乳腺癌病因的认识至关重要。肠道微生物群参与包括癌症在内的多种生理病理情况。尽管其通过改变雌激素的肠肝循环和/或植物雌激素的代谢而可能参与乳腺癌的情况已被讨论了一段时间，但仍有待证实。本研究旨在通过确定乳腺癌患者粪便微生物群组成与临床特征之间的可能联系来强化这一假设。从31例早期乳腺癌患者的粪便中提取细菌DNA，并通过实时聚合酶链反应（qPCR）进行扩增，靶向特定细菌群的16S rRNA序列，然后结合临床特征进行分析。根据患者的体重指数，总细菌以及三个细菌群（厚壁菌门、普拉梭菌和布劳特氏菌）的绝对数量存在显著差异。某些细菌群，即球状梭菌、普拉梭菌和布劳特氏菌的百分比和绝对数量，根据临床分期和组织预后分级存在显著差异。我们的研究强调，这些患者的肠道微生物群组成根据临床特征和体重指数而有所不同。需要进一步的研究来阐明乳腺癌与肠道微生物群之间的联系。
