Wyganowska-Swiatkowska Marzena, Urbaniak Paulina, Lipinski Daniel, Szalata Marlena, Borysiak Karolina, Jakun Jerzy, Kotwicka Malgorzata
Department of Conservative Dentistry and Periodontology, Collegium Stomatologicum, Poznań University of Medical Sciences, 60-812 Poznań, Poland.
Department of Cell Biology, Poznań University of Medical Sciences, 60-806 Poznań, Poland.
Exp Ther Med. 2017 Jan;13(1):160-168. doi: 10.3892/etm.2016.3918. Epub 2016 Nov 18.
Enamel matrix derivative (EMD) can mimic odontogenic effects by inducing the proliferation and differentiation of connective tissue progenitor cells, stimulating bone growth and arresting epithelial cells migration. To the best of our knowledge, there is no data indicating that any active component of EMD reduces epithelial cell viability. The present study examines the impact of commercial lyophilized EMD, porcine recombinant amelogenin (prAMEL; 21.3 kDa) and tyrosine-rich amelogenin peptide (TRAP) on the adherence, proliferation and migration of human epithelial cells in real-time. The tongue carcinoma cell line SCC-25 was stimulated with EMD, porcine recombinant AMEL and TRAP, at concentrations of 12.5, 25 and 50 µg/ml. Cell adherence, migration and proliferation were monitored in real-time using the xCELLigence system. No significant effects of EMD on the morphology, adhesion, proliferation and migration of SCC-25 cells were observed. However, porcine recombinant AMEL had a dose-dependent inhibitory effect on SCC-25 cell proliferation and migration. Predominantly, no notable differences were found between control and TRAP-treated cells in terms of cell adhesion and migration, a decrease in proliferation was observed, but this was not statistically significant. EMD and its active components do not increase the tongue cancer cell viability.
釉基质衍生物（EMD）可通过诱导结缔组织祖细胞的增殖和分化、刺激骨生长以及阻止上皮细胞迁移来模拟牙源性效应。据我们所知，尚无数据表明EMD的任何活性成分会降低上皮细胞活力。本研究实时检测了市售冻干EMD、猪重组釉原蛋白（prAMEL；21.3 kDa）和富含酪氨酸的釉原蛋白肽（TRAP）对人上皮细胞黏附、增殖和迁移的影响。用浓度为12.5、25和50 µg/ml的EMD、猪重组AMEL和TRAP刺激舌癌细胞系SCC-25。使用xCELLigence系统实时监测细胞黏附、迁移和增殖情况。未观察到EMD对SCC-25细胞的形态、黏附、增殖和迁移有显著影响。然而，猪重组AMEL对SCC-25细胞的增殖和迁移有剂量依赖性抑制作用。主要地，在细胞黏附和迁移方面，未发现对照组和TRAP处理组细胞之间有明显差异，观察到增殖有所下降，但无统计学意义。EMD及其活性成分不会增加舌癌细胞的活力。
