恒河猴胚胎干细胞来源的神经胶质限制前体细胞的特性分析。

Characterization of glial-restricted precursors from rhesus monkey embryonic stem cells.

作者信息

Chen Hongwei, Mao Yu, Wang Shufen, Li Bin, Wang Jinhuan, Li Jian, Ma Yuanye

机构信息

Laboratory of Reproductive and Developmental Biology, Kunming Primate Research Center, and Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, Yunnan 650223, P. R. China; Yunnan Key Laboratory of Animal Reproductive Biology, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, Yunnan 650223, P. R. China; Stem Cell and Brain Research Institute, INSERM U846, Bron 69675, France; Graduate University of Chinese Academy of Sciences, Beijing 100049, P. R. China.

Laboratory of Primate Recognition Neurosciences, Kunming Primate Research Center, and Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, Yunnan 650223, P. R. China; Graduate University of Chinese Academy of Sciences, Beijing 100049, P. R. China.

出版信息

Transl Neurosci. 2015 Nov 27;6(1):244-251. doi: 10.1515/tnsci-2015-0026. eCollection 2015.

DOI:10.1515/tnsci-2015-0026

PMID:28123809

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4936634/

Abstract

Glial-restricted precursor (GRP) cells, the earliest glial progenitors for both astrocytes and oligodendrocytes, have been derived from embryos and embryonic stem cells (ESC) in rodents. However, knowledge regarding the equivalent cell type in primates is limited due to restrictions imposed by ethics and resources. Here we report successful derivation and characterization of primate GRP cells from rhesus monkey ESC. The purified monkey GRP cells were AB-positive and FGF2-dependent for survival and proliferation. The differentiation assays indicated that they were tri-potential and bi-potential . These newly purified GRP cells will help to facilitate understanding of the molecular mechanism of glial development in primates as well as provide a source of therapeutic donor cells for use in neuroregenerative medicine.

摘要

胶质细胞限制前体细胞（GRP）是星形胶质细胞和少突胶质细胞最早的胶质祖细胞，已从啮齿动物的胚胎和胚胎干细胞（ESC）中获得。然而，由于伦理和资源限制，关于灵长类动物中同等细胞类型的知识有限。在此，我们报告了从恒河猴胚胎干细胞成功获得并鉴定出灵长类GRP细胞。纯化后的猴GRP细胞AB呈阳性，其存活和增殖依赖于碱性成纤维细胞生长因子2（FGF2）。分化试验表明，它们具有三潜能和双潜能。这些新纯化的GRP细胞将有助于促进对灵长类动物胶质细胞发育分子机制的理解，并为神经再生医学提供治疗性供体细胞来源。