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通过免疫沉淀法探究油菜素内酯不敏感1受体激酶的激活与失活

Probing Activation and Deactivation of the BRASSINOSTEROID INSENSITIVE1 Receptor Kinase by Immunoprecipitation.

作者信息

Martins Sara, Vert Grégory, Jaillais Yvon

机构信息

Institute for Integrative Biology of the Cell (I2BC), Centre National de la Recherche Scientifique, Commissariat à l'Energie Atomique, Université Paris Sud, Université Paris-Saclay, 91198, Gif-sur-Yvette, France.

Laboratoire de Reproduction et Développement des Plantes, Université de Lyon, Ecole Nationale Supérieure de Lyon, Université ClaudeBernard Lyon 1,, Université de Lyon, Ecole Nationale Supérieure de Lyon, Université Claude Bernard Lyon 1, Centre National de la Recherche Scientifique, Institut National de la Recheche Agronomique, 69342, Lyon, France.

出版信息

Methods Mol Biol. 2017;1564:169-180. doi: 10.1007/978-1-4939-6813-8_14.

DOI:10.1007/978-1-4939-6813-8_14
PMID:28124254
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5321509/
Abstract

Brassinosteroids (BRs) are sterol-derived hormones that control plant growth and development. The BR receptor complex is encoded by the BRASSINOSTEROID INSENSITIVE1 (BRI1) and members of the SOMATIC EMBRYOGENESIS RECEPTOR KINASE family. For activation and deactivation, the BR receptor complex uses different posttranslational modifications and recruits various partner proteins. Here, we describe optimized immunoprecipitation protocols and variants for biochemical analyses of posttranslational modifications of BRI1 and of protein-protein interactions.

摘要

油菜素类固醇（BRs）是一类由甾醇衍生而来的激素，可调控植物的生长和发育。BR受体复合物由油菜素类固醇不敏感蛋白1（BRI1）和体细胞胚胎发生受体激酶家族的成员编码。为了实现激活和失活，BR受体复合物采用不同的翻译后修饰方式，并招募各种伴侣蛋白。在此，我们描述了用于对BRI1的翻译后修饰和蛋白质-蛋白质相互作用进行生化分析的优化免疫沉淀方案及其变体。

相似文献

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Probing Activation and Deactivation of the BRASSINOSTEROID INSENSITIVE1 Receptor Kinase by Immunoprecipitation.通过免疫沉淀法探究油菜素内酯不敏感1受体激酶的激活与失活
Methods Mol Biol. 2017;1564:169-180. doi: 10.1007/978-1-4939-6813-8_14.
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本文引用的文献

1
The molecular circuitry of brassinosteroid signaling.油菜素甾醇信号传导的分子机制
New Phytol. 2015 Apr;206(2):522-40. doi: 10.1111/nph.13269. Epub 2015 Jan 23.
2
Internalization and vacuolar targeting of the brassinosteroid hormone receptor BRI1 are regulated by ubiquitination.油菜素甾体激素受体BRI1的内化和液泡靶向由泛素化调控。
Nat Commun. 2015 Jan 21;6:6151. doi: 10.1038/ncomms7151.
3
Crystal structures of the phosphorylated BRI1 kinase domain and implications for brassinosteroid signal initiation.磷酸化油菜素内酯不敏感1激酶结构域的晶体结构及其对油菜素类固醇信号起始的影响
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The brassinosteroid insensitive1-like3 signalosome complex regulates Arabidopsis root development.油菜素内酯不敏感1样3信号体复合物调控拟南芥根系发育。
Plant Cell. 2013 Sep;25(9):3377-88. doi: 10.1105/tpc.113.114462. Epub 2013 Sep 24.
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Molecular mechanism for plant steroid receptor activation by somatic embryogenesis co-receptor kinases.体细胞胚胎发生共受体激酶激活植物甾体受体的分子机制。
Science. 2013 Aug 23;341(6148):889-92. doi: 10.1126/science.1242468. Epub 2013 Aug 8.
6
Transphosphorylation of E. coli Proteins during Production of Recombinant Protein Kinases Provides a Robust System to Characterize Kinase Specificity.在重组蛋白激酶生产过程中，大肠杆菌蛋白的转磷酸化提供了一个稳健的系统来表征激酶特异性。
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7
Tyrosine Phosphorylation of the BRI1 Receptor Kinase Occurs via a Post-Translational Modification and is Activated by the Juxtamembrane Domain.BRII 受体激酶的酪氨酸磷酸化是通过翻译后修饰发生的，并被跨膜结构域激活。
Front Plant Sci. 2012 Aug 8;3:175. doi: 10.3389/fpls.2012.00175. eCollection 2012.
8
Genetic evidence for an indispensable role of somatic embryogenesis receptor kinases in brassinosteroid signaling.体细胞胚发生受体激酶在油菜素内酯信号转导中不可或缺作用的遗传证据。
PLoS Genet. 2012 Jan;8(1):e1002452. doi: 10.1371/journal.pgen.1002452. Epub 2012 Jan 12.
9
Deactivation of the Arabidopsis BRASSINOSTEROID INSENSITIVE 1 (BRI1) receptor kinase by autophosphorylation within the glycine-rich loop.拟南芥油菜素内酯不敏感 1（BRI1）受体激酶通过富含甘氨酸环内的自磷酸化失活。
Proc Natl Acad Sci U S A. 2012 Jan 3;109(1):327-32. doi: 10.1073/pnas.1108321109. Epub 2011 Dec 19.
10
Structural basis of steroid hormone perception by the receptor kinase BRI1.甾体激素感受的受体激酶 BRI1 的结构基础。
Nature. 2011 Jun 12;474(7352):467-71. doi: 10.1038/nature10153.

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