叶绿体（trnL内含子）和核（ITS）DNA序列揭示马来西亚半岛猪笼草属物种的系统发育推断。
Phylogenetic inferences of Nepenthes species in Peninsular Malaysia revealed by chloroplast (trnL intron) and nuclear (ITS) DNA sequences.
作者信息
Bunawan Hamidun, Yen Choong Chee, Yaakop Salmah, Noor Normah Mohd
机构信息
Institute of Systems Biology, Universiti Kebangsaan Malaysia (UKM), 43600, Bangi, Selangor, Malaysia.
School of Environmental and Natural Resource Sciences, Faculty of Science and Technology, Universiti Kebangsaan Malaysia (UKM), 43600, Bangi, Selangor, Malaysia.
出版信息
BMC Res Notes. 2017 Jan 26;10(1):67. doi: 10.1186/s13104-017-2379-1.
BACKGROUND
The chloroplastic trnL intron and the nuclear internal transcribed spacer (ITS) region were sequenced for 11 Nepenthes species recorded in Peninsular Malaysia to examine their phylogenetic relationship and to evaluate the usage of trnL intron and ITS sequences for phylogenetic reconstruction of this genus.
RESULTS
Phylogeny reconstruction was carried out using neighbor-joining, maximum parsimony and Bayesian analyses. All the trees revealed two major clusters, a lowland group consisting of N. ampullaria, N. mirabilis, N. gracilis and N. rafflesiana, and another containing both intermediately distributed species (N. albomarginata and N. benstonei) and four highland species (N. sanguinea, N. macfarlanei, N. ramispina and N. alba).
CONCLUSIONS
The trnL intron and ITS sequences proved to provide phylogenetic informative characters for deriving a phylogeny of Nepenthes species in Peninsular Malaysia. To our knowledge, this is the first molecular phylogenetic study of Nepenthes species occurring along an altitudinal gradient in Peninsular Malaysia.
背景
对记录在马来西亚半岛的11种猪笼草属植物的叶绿体trnL内含子和核内转录间隔区（ITS）区域进行测序，以研究它们的系统发育关系，并评估trnL内含子和ITS序列在该属系统发育重建中的应用。
结果
使用邻接法、最大简约法和贝叶斯分析进行系统发育重建。所有的树都显示出两个主要类群，一个低地类群由马来王猪笼草、奇异猪笼草、细猪笼草和莱佛士猪笼草组成，另一个类群包含中分布物种（白边猪笼草和本斯通猪笼草）和四个高地物种（血红猪笼草、麦克法兰猪笼草、刺猪笼草和白猪笼草）。
结论
trnL内含子和ITS序列被证明为推导马来西亚半岛猪笼草属物种的系统发育提供了系统发育信息特征。据我们所知，这是对马来西亚半岛沿海拔梯度分布的猪笼草属物种的首次分子系统发育研究。
Zotero 插件