Keen C L, Lönnerdal B, Golub M S, Uriu-Hare J Y, Olin K L, Hendrickx A G, Gershwin M E
Department of Nutrition, University of California, Davis 95616.
Pediatr Res. 1989 Nov;26(5):470-7. doi: 10.1203/00006450-198911000-00022.
To investigate the effects of marginal zinc deficiency on early development, rhesus monkeys were fed a diet marginally deficient in zinc (M; 4 micrograms/g) throughout pregnancy and during the first month of lactation. Despite the low concentration of zinc in the diet. M dams did not develop overt signs of zinc deficiency. However, compared to control dams fed diets adequate in zinc (C; 100 micrograms Zn/g), M dams showed a low response to the mitogens concanavalin A and phytohemagglutinin. Pregnancy outcome was similar in the two groups and all of the neonates were judged to be healthy at delivery. From birth until d 30 of age, the infants were closely monitored for signs of zinc deficiency, and at d 30, they were killed and tissues were removed and analyzed for a number of parameters reported to be affected by zinc status. At birth, M infants had low plasma zinc concentrations compared to controls; however, this difference was not observed at d 30. D 30 M infants showed a normal response to the mitogens concanavalin A and phytohemagglutinin, but showed a low response to pokeweed mitogen. Tissue (liver, brain, spleen, kidney, and heart) trace element concentrations were similar in the two groups of infants, as were liver metallothionein concentrations and 65Zn uptake/retention by isolated hepatocytes. Infant wt gain was inversely correlated with plasma zinc, liver zinc, and liver metallothionein concentrations in both the M and C groups.(ABSTRACT TRUNCATED AT 250 WORDS)
为研究边缘性锌缺乏对早期发育的影响，在整个孕期及哺乳期的第一个月，给恒河猴喂食边缘性锌缺乏饮食（M组；4微克/克）。尽管饮食中锌浓度较低，但M组母猴未出现明显的锌缺乏迹象。然而，与喂食锌充足饮食（C组；100微克锌/克）的对照母猴相比，M组母猴对促细胞分裂剂刀豆球蛋白A和植物血凝素的反应较低。两组的妊娠结局相似，所有新生儿在出生时均被判定为健康。从出生到30日龄，密切监测婴儿是否有锌缺乏迹象，在30日龄时，将其处死，取出组织并分析一些据报道受锌状态影响的参数。出生时，M组婴儿的血浆锌浓度低于对照组；然而，在30日龄时未观察到这种差异。30日龄的M组婴儿对刀豆球蛋白A和植物血凝素的反应正常，但对商陆促细胞分裂剂的反应较低。两组婴儿组织（肝脏、大脑、脾脏、肾脏和心脏）中的微量元素浓度相似，肝脏金属硫蛋白浓度以及分离的肝细胞对65Zn的摄取/保留情况也相似。M组和C组婴儿的体重增加均与血浆锌、肝脏锌和肝脏金属硫蛋白浓度呈负相关。（摘要截选至250词）
