前列腺素F2α在体外与牛眼黑色素及合成黑色素的结合

Prostaglandin F2 alpha binding to bovine ocular and synthetic melanins in vitro.

作者信息

Aula P, Kaila T, Huupponen R, Salminen L, Iisalo E

机构信息

Department of Pharmacology, University of Turku, Finland.

出版信息

Pharmacol Toxicol. 1989 Aug;65(2):100-3. doi: 10.1111/j.1600-0773.1989.tb01136.x.

DOI:10.1111/j.1600-0773.1989.tb01136.x

PMID:2813281

Abstract

The binding of 3H-prostaglandin F2 alpha to bovine iris and synthetic melanin was studied in vitro using a ligand binding assay. Prostaglandin F2 alpha was reversibly bound to both types of melanin. The binding was saturable and the Scatchard analysis revealed the existence of at least two binding sites with the corresponding K(D) values of 3.71 nM and 1.99 microM for natural and 4.99 nM and 0.19 microM for synthetic melanin, respectively. The high affinity Bmax values were 3.4 nM/g for natural and 2.5 nM/g for synthetic melanin. The dissociation of prostaglandin F2 alpha from melanin after dilution of the complex was rapid and uniform.

摘要

利用配体结合试验在体外研究了³H - 前列腺素F2α与牛虹膜及合成黑色素的结合情况。前列腺素F2α与两种类型的黑色素均呈可逆性结合。这种结合具有饱和性，Scatchard分析表明天然黑色素存在至少两个结合位点，其相应的解离常数（K(D)）值分别为3.71 nM和1.99 μM，合成黑色素的K(D)值分别为4.99 nM和0.19 μM。天然黑色素的高亲和力最大结合量（Bmax）值为3.4 nM/g，合成黑色素的为2.5 nM/g。复合物稀释后，前列腺素F2α从黑色素上的解离迅速且均匀。