Masuda Hayato, Horie Takafumi, Hubacz Robert, Ohmura Naoto, Shimoyamada Makoto
a School of Food and Nutritional Science, University of Shizuoka , Shizuoka , Japan.
b Complex Fluid and Thermal Engineering Research Center (COFTEC), Kobe University , Kobe , Japan.
Biosci Biotechnol Biochem. 2017 Apr;81(4):755-761. doi: 10.1080/09168451.2017.1282806. Epub 2017 Feb 1.
In food industries, enzymatic starch hydrolysis is an important process that consists of two steps: gelatinization and saccharification. One of the major difficulties in designing the starch hydrolysis process is the sharp change in its rheological properties. In this study, Taylor-Couette flow reactor was applied to continuous starch hydrolysis process. The concentration of reducing sugar produced via enzymatic hydrolysis was evaluated by varying operational variables: rotational speed of the inner cylinder, axial velocity (reaction time), amount of enzyme, and initial starch content in the slurry. When Taylor vortices were formed in the annular space, efficient hydrolysis occurred because Taylor vortices improved the mixing of gelatinized starch with enzyme. Furthermore, a modified inner cylinder was proposed, and its mixing performance was numerically investigated. The modified inner cylinder showed higher potential for enhanced mixing of gelatinized starch and the enzyme than the conventional cylinder.
在食品工业中,酶促淀粉水解是一个重要过程,它包括两个步骤:糊化和糖化。设计淀粉水解过程的主要困难之一是其流变特性的急剧变化。在本研究中,泰勒-库埃特流动反应器被应用于连续淀粉水解过程。通过改变操作变量来评估酶促水解产生的还原糖浓度:内筒转速、轴向速度(反应时间)、酶量和浆料中的初始淀粉含量。当在环形空间中形成泰勒涡时,由于泰勒涡改善了糊化淀粉与酶的混合,从而发生了高效水解。此外,提出了一种改进的内筒,并对其混合性能进行了数值研究。与传统内筒相比,改进后的内筒在增强糊化淀粉和酶的混合方面具有更高的潜力。