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黄腹海蛇(有鳞目:眼镜蛇科)睾丸内精子细胞发育的超微结构

Ultrastructure of spermatid development within the testis of the Yellow-Bellied Sea Snake, (Squamata: Elapidae).

作者信息

Gribbins Kevin M, Freeborn Layla R, Sever David M

机构信息

Department of Biology, University of Indianapolis , Indianapolis, IN, USA.

Department of Biological Sciences, University of Pittsburgh , Pittsburgh, PA, USA.

出版信息

Spermatogenesis. 2016 Nov 18;6(3):e1261666. doi: 10.1080/21565562.2016.1261666. eCollection 2016.

Abstract

Little is known about spermatid development during spermiogenesis in snakes, as there is only one complete study in ophidians, which details the spermatid ultrastructure within the viperid, . Thus, the following study will add to our understanding of the ontogenic steps of spermiogenesis in snakes by examining spermatid maturation in the elapid, , which were collected in Costa Rica in 2009. The spermatids of share many similar ultrastructural characteristics to that described for other squamates during spermiogenesis. Three notable differences between the spermatids of and those of other snakes is a round and shorter epinuclear lucent zone, enlarged caudal nuclear shoulders, and more prominent 3 and 8 peripheral fibers in the principal and endpieces. Also, the midpiece is much longer in and is similar to that reported for all snakes studied to date. Other features of chromatin condensation and morphology of the acrosome complex are similar to what has been observed in and other squamates. Though the spermatids in appear to be quite similar to other snakes and lizards studied to date, some differences in subcellular details are still observed. Analysis of developing spermatids in and other snakes could reveals morphologically conserved traits between different species along with subtle changes that could help determine phylogenetic relationships once a suitable number of species have been examined for ophidians and other squamates.

摘要

关于蛇精子发生过程中精子细胞的发育,我们所知甚少,因为在蛇类中仅有一项完整的研究,该研究详细描述了蝰蛇科动物精子细胞的超微结构。因此,以下研究将通过检查眼镜蛇科动物(于2009年在哥斯达黎加采集)精子细胞的成熟过程,增进我们对蛇精子发生个体发育步骤的理解。[具体蛇类名称]的精子细胞在精子发生过程中与其他有鳞目动物所描述的具有许多相似的超微结构特征。[具体蛇类名称]的精子细胞与其他蛇类的精子细胞之间有三个显著差异,即核上透明区呈圆形且较短、尾侧核肩增大以及主段和末段中3和8条外周纤维更突出。此外,[具体蛇类名称]的中段长得多,与迄今研究的所有蛇类报道的情况相似。染色质浓缩和顶体复合体形态的其他特征与在[具体蛇类名称]和其他有鳞目动物中观察到的情况相似。尽管[具体蛇类名称]的精子细胞似乎与迄今研究的其他蛇类和蜥蜴非常相似,但仍观察到一些亚细胞细节上的差异。对[具体蛇类名称]和其他蛇类发育中的精子细胞进行分析,可能会揭示不同物种之间形态上保守的特征以及细微变化,一旦对蛇类和其他有鳞目动物进行了适当数量物种的研究,这些变化可能有助于确定系统发育关系。

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